Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation
Proteins of Atlantic salmon (Salmo salar L.) spermatozoa were lost into the seminal fluid during storage at −80 °C. Addition of glycerol or dimethylsulfoxide reduced but did not completely prevent loss of sperm proteins. Of the two, glycerol more effectively fostered retention of sperm proteins, esp...
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Canadian Science Publishing
1987
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Online Access: | http://dx.doi.org/10.1139/z87-002 http://www.nrcresearchpress.com/doi/pdf/10.1139/z87-002 |
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crcansciencepubl:10.1139/z87-002 2023-12-17T10:27:14+01:00 Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation Yoo, Bong Y. Ryan, Mary A. Wiggs, A. James 1987 http://dx.doi.org/10.1139/z87-002 http://www.nrcresearchpress.com/doi/pdf/10.1139/z87-002 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 65, issue 1, page 9-13 ISSN 0008-4301 1480-3283 Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 1987 crcansciencepubl https://doi.org/10.1139/z87-002 2023-11-19T13:39:29Z Proteins of Atlantic salmon (Salmo salar L.) spermatozoa were lost into the seminal fluid during storage at −80 °C. Addition of glycerol or dimethylsulfoxide reduced but did not completely prevent loss of sperm proteins. Of the two, glycerol more effectively fostered retention of sperm proteins, especially those of higher molecular weight. Some of the proteins lost during storage were surface labelled via lactoperoxidase-mediated iodination. More protein species were labelled in frozen than in fresh spermatozoa. The massive loss and extensive labelling of sperm proteins with 125 I are taken together as an indication of damage to salmon sperm membranes during freezing, storage, and thawing. Article in Journal/Newspaper Atlantic salmon Salmo salar Canadian Science Publishing (via Crossref) Canadian Journal of Zoology 65 1 9 13 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Yoo, Bong Y. Ryan, Mary A. Wiggs, A. James Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
topic_facet |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
description |
Proteins of Atlantic salmon (Salmo salar L.) spermatozoa were lost into the seminal fluid during storage at −80 °C. Addition of glycerol or dimethylsulfoxide reduced but did not completely prevent loss of sperm proteins. Of the two, glycerol more effectively fostered retention of sperm proteins, especially those of higher molecular weight. Some of the proteins lost during storage were surface labelled via lactoperoxidase-mediated iodination. More protein species were labelled in frozen than in fresh spermatozoa. The massive loss and extensive labelling of sperm proteins with 125 I are taken together as an indication of damage to salmon sperm membranes during freezing, storage, and thawing. |
format |
Article in Journal/Newspaper |
author |
Yoo, Bong Y. Ryan, Mary A. Wiggs, A. James |
author_facet |
Yoo, Bong Y. Ryan, Mary A. Wiggs, A. James |
author_sort |
Yoo, Bong Y. |
title |
Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
title_short |
Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
title_full |
Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
title_fullStr |
Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
title_full_unstemmed |
Loss of protein from spermatozoa of Atlantic salmon ( Salmo salar L.) because of cryopreservation |
title_sort |
loss of protein from spermatozoa of atlantic salmon ( salmo salar l.) because of cryopreservation |
publisher |
Canadian Science Publishing |
publishDate |
1987 |
url |
http://dx.doi.org/10.1139/z87-002 http://www.nrcresearchpress.com/doi/pdf/10.1139/z87-002 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Canadian Journal of Zoology volume 65, issue 1, page 9-13 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z87-002 |
container_title |
Canadian Journal of Zoology |
container_volume |
65 |
container_issue |
1 |
container_start_page |
9 |
op_container_end_page |
13 |
_version_ |
1785579037526065152 |