Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae)
Laboratory methods were developed for culturing the free-living stages of Neomesomermis flumenalis from field-collected blackfly larvae. The best results were obtained by placing 50 pairs of nematodes under xenic conditions in 500-ml containers containing about 450 ml of wet sand. With this method,...
Published in: | Canadian Journal of Zoology |
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Language: | English |
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Canadian Science Publishing
1977
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Online Access: | http://dx.doi.org/10.1139/z77-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/z77-053 |
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crcansciencepubl:10.1139/z77-053 2023-12-17T10:44:55+01:00 Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) Bailey, C. H. Gordon, R. Mills, C. 1977 http://dx.doi.org/10.1139/z77-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/z77-053 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 55, issue 2, page 391-397 ISSN 0008-4301 1480-3283 Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 1977 crcansciencepubl https://doi.org/10.1139/z77-053 2023-11-19T13:38:34Z Laboratory methods were developed for culturing the free-living stages of Neomesomermis flumenalis from field-collected blackfly larvae. The best results were obtained by placing 50 pairs of nematodes under xenic conditions in 500-ml containers containing about 450 ml of wet sand. With this method, 78.6% of the females introduced to culture reproduced. The influence of factors such as microorganisms, substrate, container volume, and sterility on culturing these mermithids is discussed. Differences were noted in the development of N. flumenalis from winter-breeding Prosimulium hosts and those from summer-breeding Simulium hosts. Parasites from Simulium developed nearly twice as fast as from Prosimulium hosts at 10 °C. Temperature studies showed that Simulium parasites were better adapted for higher temperature ranges (20 °C) but were more poorly adapted to lower temperatures (5 °C) than parasites from Prosimulium. The noted differences suggest two physiological strains of the same species, one developing in Simulium spp., the other in Prosimulium spp. Article in Journal/Newspaper Newfoundland Canadian Science Publishing (via Crossref) Canadian Journal of Zoology 55 2 391 397 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Bailey, C. H. Gordon, R. Mills, C. Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
topic_facet |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
description |
Laboratory methods were developed for culturing the free-living stages of Neomesomermis flumenalis from field-collected blackfly larvae. The best results were obtained by placing 50 pairs of nematodes under xenic conditions in 500-ml containers containing about 450 ml of wet sand. With this method, 78.6% of the females introduced to culture reproduced. The influence of factors such as microorganisms, substrate, container volume, and sterility on culturing these mermithids is discussed. Differences were noted in the development of N. flumenalis from winter-breeding Prosimulium hosts and those from summer-breeding Simulium hosts. Parasites from Simulium developed nearly twice as fast as from Prosimulium hosts at 10 °C. Temperature studies showed that Simulium parasites were better adapted for higher temperature ranges (20 °C) but were more poorly adapted to lower temperatures (5 °C) than parasites from Prosimulium. The noted differences suggest two physiological strains of the same species, one developing in Simulium spp., the other in Prosimulium spp. |
format |
Article in Journal/Newspaper |
author |
Bailey, C. H. Gordon, R. Mills, C. |
author_facet |
Bailey, C. H. Gordon, R. Mills, C. |
author_sort |
Bailey, C. H. |
title |
Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
title_short |
Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
title_full |
Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
title_fullStr |
Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
title_full_unstemmed |
Laboratory culture of the free-living stages of Neomesomermis flumenalis , a mermithid nematode parasite of Newfoundland blackflies (Diptera: Simuliidae) |
title_sort |
laboratory culture of the free-living stages of neomesomermis flumenalis , a mermithid nematode parasite of newfoundland blackflies (diptera: simuliidae) |
publisher |
Canadian Science Publishing |
publishDate |
1977 |
url |
http://dx.doi.org/10.1139/z77-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/z77-053 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Canadian Journal of Zoology volume 55, issue 2, page 391-397 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z77-053 |
container_title |
Canadian Journal of Zoology |
container_volume |
55 |
container_issue |
2 |
container_start_page |
391 |
op_container_end_page |
397 |
_version_ |
1785564471086809088 |