mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada
Two nominal species of freshwater mussels, Pyganodon cataracta (Say, 1817) (eastern floater) and Pyganodon fragilis (Lamarck, 1819) (Newfoundland floater), occur in Atlantic Canada, but their taxonomic status has been controversial. We analyzed the female-transmitted mitochondrial cytochrome c oxida...
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crcansciencepubl:10.1139/z2012-100 2023-12-17T10:44:56+01:00 mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada Stanton, L.M. Hoeh, W.R. McAlpine, D.F. Hebda, A. Stewart, D.T. 2012 http://dx.doi.org/10.1139/z2012-100 http://www.nrcresearchpress.com/doi/full-xml/10.1139/z2012-100 http://www.nrcresearchpress.com/doi/pdf/10.1139/z2012-100 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 90, issue 11, page 1307-1319 ISSN 0008-4301 1480-3283 Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 2012 crcansciencepubl https://doi.org/10.1139/z2012-100 2023-11-19T13:38:54Z Two nominal species of freshwater mussels, Pyganodon cataracta (Say, 1817) (eastern floater) and Pyganodon fragilis (Lamarck, 1819) (Newfoundland floater), occur in Atlantic Canada, but their taxonomic status has been controversial. We analyzed the female-transmitted mitochondrial cytochrome c oxidase subunit I (i.e., FCOI) and nuclear internal transcribed spacer region (ITS-1) gene sequences, as well as multilocus amplified fragment length polymorphism (AFLP) markers, to evaluate genetic variation in Pyganodon specimens from Atlantic Canada. We found that Pyganodon samples from this region displayed no or extremely low (i.e., <0.5%) genetic divergence for ITS-1 and FCOI sequence data, respectively. Inferences from nested clade analysis of FCOI haplotypes suggest restricted gene flow with isolation by distance and contiguous range expansion. Analysis of molecular variance inferred from presence or absence of nuclear-encoded AFLP bands showed moderate geographic structuring among provinces but no correspondence between mtDNA haplotypes and AFLP profiles. Similarly, there was no correspondence between presence of a single or a double loop on the umbo and FCOI haplotype. These mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta–Pyganodon fragilis freshwater mussel complex in Atlantic Canada and question the species-level distinctness of these putative taxa. Article in Journal/Newspaper Newfoundland Canadian Science Publishing (via Crossref) Canada Lamarck ENVELOPE(140.027,140.027,-66.666,-66.666) Canadian Journal of Zoology 90 11 1307 1319 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Stanton, L.M. Hoeh, W.R. McAlpine, D.F. Hebda, A. Stewart, D.T. mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
topic_facet |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
description |
Two nominal species of freshwater mussels, Pyganodon cataracta (Say, 1817) (eastern floater) and Pyganodon fragilis (Lamarck, 1819) (Newfoundland floater), occur in Atlantic Canada, but their taxonomic status has been controversial. We analyzed the female-transmitted mitochondrial cytochrome c oxidase subunit I (i.e., FCOI) and nuclear internal transcribed spacer region (ITS-1) gene sequences, as well as multilocus amplified fragment length polymorphism (AFLP) markers, to evaluate genetic variation in Pyganodon specimens from Atlantic Canada. We found that Pyganodon samples from this region displayed no or extremely low (i.e., <0.5%) genetic divergence for ITS-1 and FCOI sequence data, respectively. Inferences from nested clade analysis of FCOI haplotypes suggest restricted gene flow with isolation by distance and contiguous range expansion. Analysis of molecular variance inferred from presence or absence of nuclear-encoded AFLP bands showed moderate geographic structuring among provinces but no correspondence between mtDNA haplotypes and AFLP profiles. Similarly, there was no correspondence between presence of a single or a double loop on the umbo and FCOI haplotype. These mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta–Pyganodon fragilis freshwater mussel complex in Atlantic Canada and question the species-level distinctness of these putative taxa. |
format |
Article in Journal/Newspaper |
author |
Stanton, L.M. Hoeh, W.R. McAlpine, D.F. Hebda, A. Stewart, D.T. |
author_facet |
Stanton, L.M. Hoeh, W.R. McAlpine, D.F. Hebda, A. Stewart, D.T. |
author_sort |
Stanton, L.M. |
title |
mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
title_short |
mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
title_full |
mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
title_fullStr |
mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
title_full_unstemmed |
mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataracta – Pyganodon fragilis freshwater mussel complex in Atlantic Canada |
title_sort |
mtdna and aflp markers demonstrate limited genetic differentiation within the pyganodon cataracta – pyganodon fragilis freshwater mussel complex in atlantic canada |
publisher |
Canadian Science Publishing |
publishDate |
2012 |
url |
http://dx.doi.org/10.1139/z2012-100 http://www.nrcresearchpress.com/doi/full-xml/10.1139/z2012-100 http://www.nrcresearchpress.com/doi/pdf/10.1139/z2012-100 |
long_lat |
ENVELOPE(140.027,140.027,-66.666,-66.666) |
geographic |
Canada Lamarck |
geographic_facet |
Canada Lamarck |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Canadian Journal of Zoology volume 90, issue 11, page 1307-1319 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z2012-100 |
container_title |
Canadian Journal of Zoology |
container_volume |
90 |
container_issue |
11 |
container_start_page |
1307 |
op_container_end_page |
1319 |
_version_ |
1785564556199723008 |