mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples
Whether gene-sequence information could be obtained using mRNA from road-kill and hunting samples was investigated. Adipose tissue was used to clone cDNA fragments of the hormone leptin and brain tissue was used for the enzyme glutamic acid decarboxylase (GAD). Tissues collected from road-killed ani...
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2003
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Online Access: | http://dx.doi.org/10.1139/z03-048 http://www.nrcresearchpress.com/doi/pdf/10.1139/z03-048 |
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crcansciencepubl:10.1139/z03-048 2023-12-17T10:18:02+01:00 mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples Doyon, C Trudeau, V L Hibbert, B M Howes, L A Moon, T W 2003 http://dx.doi.org/10.1139/z03-048 http://www.nrcresearchpress.com/doi/pdf/10.1139/z03-048 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Zoology volume 81, issue 4, page 692-698 ISSN 0008-4301 1480-3283 Animal Science and Zoology Ecology, Evolution, Behavior and Systematics journal-article 2003 crcansciencepubl https://doi.org/10.1139/z03-048 2023-11-19T13:39:17Z Whether gene-sequence information could be obtained using mRNA from road-kill and hunting samples was investigated. Adipose tissue was used to clone cDNA fragments of the hormone leptin and brain tissue was used for the enzyme glutamic acid decarboxylase (GAD). Tissues collected from road-killed animals were used to clone leptin from RNA samples of raccoon (Procyon lotor) and woodchuck (Marmota monax). We were able to extract RNA and clone GAD67 from samples of masked shrew (Sorex cinereus), although the time of death was unknown. We collaborated with hunters who provided tissues from which we cloned leptin and GAD isoforms from beaver (Castor canadensis), red squirrel (Tamiasciurus hudsonicus), black bear (Ursus americanus), and moose (Alces alces americana). Molecular phylogenetic analyses confirmed that the sequences obtained did not result from contamination. A time-course experiment showed that even 24 h after the death of rats, sufficient mRNA remains to amplify leptin from adipose tissue. These results suggest that road-kill and hunting samples could be used as a valuable source of gene-sequence information. Article in Journal/Newspaper Alces alces Canadian Science Publishing (via Crossref) Canadian Journal of Zoology 81 4 692 698 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Doyon, C Trudeau, V L Hibbert, B M Howes, L A Moon, T W mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
topic_facet |
Animal Science and Zoology Ecology, Evolution, Behavior and Systematics |
description |
Whether gene-sequence information could be obtained using mRNA from road-kill and hunting samples was investigated. Adipose tissue was used to clone cDNA fragments of the hormone leptin and brain tissue was used for the enzyme glutamic acid decarboxylase (GAD). Tissues collected from road-killed animals were used to clone leptin from RNA samples of raccoon (Procyon lotor) and woodchuck (Marmota monax). We were able to extract RNA and clone GAD67 from samples of masked shrew (Sorex cinereus), although the time of death was unknown. We collaborated with hunters who provided tissues from which we cloned leptin and GAD isoforms from beaver (Castor canadensis), red squirrel (Tamiasciurus hudsonicus), black bear (Ursus americanus), and moose (Alces alces americana). Molecular phylogenetic analyses confirmed that the sequences obtained did not result from contamination. A time-course experiment showed that even 24 h after the death of rats, sufficient mRNA remains to amplify leptin from adipose tissue. These results suggest that road-kill and hunting samples could be used as a valuable source of gene-sequence information. |
format |
Article in Journal/Newspaper |
author |
Doyon, C Trudeau, V L Hibbert, B M Howes, L A Moon, T W |
author_facet |
Doyon, C Trudeau, V L Hibbert, B M Howes, L A Moon, T W |
author_sort |
Doyon, C |
title |
mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
title_short |
mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
title_full |
mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
title_fullStr |
mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
title_full_unstemmed |
mRNA analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
title_sort |
mrna analysis in flattened fauna: obtaining gene-sequence information from road-kill and game-hunting samples |
publisher |
Canadian Science Publishing |
publishDate |
2003 |
url |
http://dx.doi.org/10.1139/z03-048 http://www.nrcresearchpress.com/doi/pdf/10.1139/z03-048 |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Canadian Journal of Zoology volume 81, issue 4, page 692-698 ISSN 0008-4301 1480-3283 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/z03-048 |
container_title |
Canadian Journal of Zoology |
container_volume |
81 |
container_issue |
4 |
container_start_page |
692 |
op_container_end_page |
698 |
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1785537928928165888 |