Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats
Administration of a Prudhoe Bay crude oil (PBCO) to pregnant rats resulted in induction of hepatic microsomal P-450 levels and various monooxygenases in a dose-dependent manner. The activities of aniline hydroxylase, benzo[a]pyrene hydroxylase, aminopyrine-N-demethylase, ethoxyresorufin-O-deethylase...
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1987
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crcansciencepubl:10.1139/y87-381 2024-06-23T07:56:20+00:00 Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats Khan, Sumsullah Martin, Marie Rahimtula, Anver D. Payne, Jeremiah F. 1987 http://dx.doi.org/10.1139/y87-381 http://www.nrcresearchpress.com/doi/pdf/10.1139/y87-381 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Physiology and Pharmacology volume 65, issue 12, page 2400-2408 ISSN 0008-4212 1205-7541 journal-article 1987 crcansciencepubl https://doi.org/10.1139/y87-381 2024-05-30T08:13:48Z Administration of a Prudhoe Bay crude oil (PBCO) to pregnant rats resulted in induction of hepatic microsomal P-450 levels and various monooxygenases in a dose-dependent manner. The activities of aniline hydroxylase, benzo[a]pyrene hydroxylase, aminopyrine-N-demethylase, ethoxyresorufin-O-deethylase, and pentoxyresorufin-O-depentylase were increased 2–3-fold, 12–15-fold, 1.4–1.8-fold, 20–24-fold, and 6–8-fold, respectively, on gestation day 18, when a single dose of PBCO (5–10 mL/kg body weight, p.o.) had been administered 24 h earlier. Glutathione-S-transferase, UDPG transferase, and DT-diaphorase activities were also increased; however, maximum induction was noticed when crude oil was given 72 h earlier. Repeated exposure (day 6 – day 17, daily) of crude oil at lower levels was able to produce similar induction patterns in enzyme systems at day 18 of gestation. The xenobiotic-metabolizing enzyme systems were also induced transplacentally: treatment of pregnant rats with PBCO induced both placental and fetal hepatic enzyme systems. Liver microsomal P-450 contents, benzo[a]pyrene hydroxylase, and ethoxyresorufin-O-deethylase activities were increased 2-fold, 2–3-fold, and 10–12-fold, respectively in 18-day-old fetuses. Similar trends were noticed in placenta. Activities of phase II enzymes such as glutathione-S-transferase, UDPG transferase, and DT-diaphorase were also significantly elevated. It is suggested that crude oil induces maternal hepatic drug metabolism and that some of its constituents (mainly aromatic hydrocarbons) and (or) their metabolites pass through the placenta and thus induce drug-metabolizing enzymes transplacentally. The practical importance of the results in relation to human and environmental health is also discussed. Article in Journal/Newspaper Prudhoe Bay Canadian Science Publishing Canadian Journal of Physiology and Pharmacology 65 12 2400 2408 |
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Canadian Science Publishing |
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Administration of a Prudhoe Bay crude oil (PBCO) to pregnant rats resulted in induction of hepatic microsomal P-450 levels and various monooxygenases in a dose-dependent manner. The activities of aniline hydroxylase, benzo[a]pyrene hydroxylase, aminopyrine-N-demethylase, ethoxyresorufin-O-deethylase, and pentoxyresorufin-O-depentylase were increased 2–3-fold, 12–15-fold, 1.4–1.8-fold, 20–24-fold, and 6–8-fold, respectively, on gestation day 18, when a single dose of PBCO (5–10 mL/kg body weight, p.o.) had been administered 24 h earlier. Glutathione-S-transferase, UDPG transferase, and DT-diaphorase activities were also increased; however, maximum induction was noticed when crude oil was given 72 h earlier. Repeated exposure (day 6 – day 17, daily) of crude oil at lower levels was able to produce similar induction patterns in enzyme systems at day 18 of gestation. The xenobiotic-metabolizing enzyme systems were also induced transplacentally: treatment of pregnant rats with PBCO induced both placental and fetal hepatic enzyme systems. Liver microsomal P-450 contents, benzo[a]pyrene hydroxylase, and ethoxyresorufin-O-deethylase activities were increased 2-fold, 2–3-fold, and 10–12-fold, respectively in 18-day-old fetuses. Similar trends were noticed in placenta. Activities of phase II enzymes such as glutathione-S-transferase, UDPG transferase, and DT-diaphorase were also significantly elevated. It is suggested that crude oil induces maternal hepatic drug metabolism and that some of its constituents (mainly aromatic hydrocarbons) and (or) their metabolites pass through the placenta and thus induce drug-metabolizing enzymes transplacentally. The practical importance of the results in relation to human and environmental health is also discussed. |
format |
Article in Journal/Newspaper |
author |
Khan, Sumsullah Martin, Marie Rahimtula, Anver D. Payne, Jeremiah F. |
spellingShingle |
Khan, Sumsullah Martin, Marie Rahimtula, Anver D. Payne, Jeremiah F. Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
author_facet |
Khan, Sumsullah Martin, Marie Rahimtula, Anver D. Payne, Jeremiah F. |
author_sort |
Khan, Sumsullah |
title |
Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
title_short |
Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
title_full |
Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
title_fullStr |
Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
title_full_unstemmed |
Effect of a Prudhoe Bay crude oil on hepatic and placental drug metabolism in rats |
title_sort |
effect of a prudhoe bay crude oil on hepatic and placental drug metabolism in rats |
publisher |
Canadian Science Publishing |
publishDate |
1987 |
url |
http://dx.doi.org/10.1139/y87-381 http://www.nrcresearchpress.com/doi/pdf/10.1139/y87-381 |
genre |
Prudhoe Bay |
genre_facet |
Prudhoe Bay |
op_source |
Canadian Journal of Physiology and Pharmacology volume 65, issue 12, page 2400-2408 ISSN 0008-4212 1205-7541 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/y87-381 |
container_title |
Canadian Journal of Physiology and Pharmacology |
container_volume |
65 |
container_issue |
12 |
container_start_page |
2400 |
op_container_end_page |
2408 |
_version_ |
1802649371417772032 |