In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure
We examined in vitro vascular reactivity of eels previously acclimatized to 10.1 MPa hydrostatic pressure (HP) for 21 days. The isometric tension developed by ventral aortic rings was measured at atmospheric pressure. Dose-response curves for either acetylcholine (ACh) or noradrenaline (NA), as well...
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crcansciencepubl:10.1139/y00-074 2023-12-17T10:18:35+01:00 In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure Guerrero, François Theron, Mickael Sebert, Philippe 2000 http://dx.doi.org/10.1139/y00-074 http://www.nrcresearchpress.com/doi/pdf/10.1139/y00-074 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Physiology and Pharmacology volume 78, issue 11, page 897-903 ISSN 0008-4212 1205-7541 Physiology (medical) Pharmacology General Medicine Physiology journal-article 2000 crcansciencepubl https://doi.org/10.1139/y00-074 2023-11-19T13:38:21Z We examined in vitro vascular reactivity of eels previously acclimatized to 10.1 MPa hydrostatic pressure (HP) for 21 days. The isometric tension developed by ventral aortic rings was measured at atmospheric pressure. Dose-response curves for either acetylcholine (ACh) or noradrenaline (NA), as well as contractions evoked by 80 mM K + , were compared with time-matched experiments conducted on rings obtained from control eels. Results showed that neither the optimal tension nor the maximal force of the K + -evoked contraction were significantly modified, suggesting that acclimatization to high HP did not change the vascular smooth muscle contractile machinery. The dose-response curve to ACh was not significantly changed. Conversely, although NA always relaxed aortic rings, the response of acclimatized eels was significantly reduced over the entire range of the agonist concentration tested (10 -8 to 10 -3 M), except for the lowest one (10 -9 M). The maximal amplitude of the NA-induced relaxation was significantly reduced in aortic rings from acclimatized eels as compared with non-acclimatized samples (339.3 ± 86.5 vs. 744.3 ± 72.1 mg·mg -1 dry weight, P < 0.005). Our results suggest that acclimatization to high HP could selectively alter the control of vascular tone by catecholamines.Key words: fish, high pressure, vascular smooth muscle, adrenoceptors, cholinergic receptors. Article in Journal/Newspaper Anguilla anguilla Canadian Science Publishing (via Crossref) Canadian Journal of Physiology and Pharmacology 78 11 897 903 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
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English |
topic |
Physiology (medical) Pharmacology General Medicine Physiology |
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Physiology (medical) Pharmacology General Medicine Physiology Guerrero, François Theron, Mickael Sebert, Philippe In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
topic_facet |
Physiology (medical) Pharmacology General Medicine Physiology |
description |
We examined in vitro vascular reactivity of eels previously acclimatized to 10.1 MPa hydrostatic pressure (HP) for 21 days. The isometric tension developed by ventral aortic rings was measured at atmospheric pressure. Dose-response curves for either acetylcholine (ACh) or noradrenaline (NA), as well as contractions evoked by 80 mM K + , were compared with time-matched experiments conducted on rings obtained from control eels. Results showed that neither the optimal tension nor the maximal force of the K + -evoked contraction were significantly modified, suggesting that acclimatization to high HP did not change the vascular smooth muscle contractile machinery. The dose-response curve to ACh was not significantly changed. Conversely, although NA always relaxed aortic rings, the response of acclimatized eels was significantly reduced over the entire range of the agonist concentration tested (10 -8 to 10 -3 M), except for the lowest one (10 -9 M). The maximal amplitude of the NA-induced relaxation was significantly reduced in aortic rings from acclimatized eels as compared with non-acclimatized samples (339.3 ± 86.5 vs. 744.3 ± 72.1 mg·mg -1 dry weight, P < 0.005). Our results suggest that acclimatization to high HP could selectively alter the control of vascular tone by catecholamines.Key words: fish, high pressure, vascular smooth muscle, adrenoceptors, cholinergic receptors. |
format |
Article in Journal/Newspaper |
author |
Guerrero, François Theron, Mickael Sebert, Philippe |
author_facet |
Guerrero, François Theron, Mickael Sebert, Philippe |
author_sort |
Guerrero, François |
title |
In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
title_short |
In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
title_full |
In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
title_fullStr |
In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
title_full_unstemmed |
In vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( Anguilla anguilla L.) acclimatized to 10.1 MPa hydrostatic pressure |
title_sort |
in vitro reactivity of ventral aorta to acetylcholine and noradrenaline in yellow freshwater eel ( anguilla anguilla l.) acclimatized to 10.1 mpa hydrostatic pressure |
publisher |
Canadian Science Publishing |
publishDate |
2000 |
url |
http://dx.doi.org/10.1139/y00-074 http://www.nrcresearchpress.com/doi/pdf/10.1139/y00-074 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Canadian Journal of Physiology and Pharmacology volume 78, issue 11, page 897-903 ISSN 0008-4212 1205-7541 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/y00-074 |
container_title |
Canadian Journal of Physiology and Pharmacology |
container_volume |
78 |
container_issue |
11 |
container_start_page |
897 |
op_container_end_page |
903 |
_version_ |
1785577847686955008 |