Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism
The physical mapping of genes can reveal the organization of a genome and identify relationships of plant species, especially where they are involved in interspecific hybridization and polyploidy. Here we determine the chromosomal locations of the major ribosomal gene family (18S-5.8S-26S rDNA) by f...
Published in: | Canadian Journal of Forest Research |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Canadian Science Publishing
1995
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1139/x95-012 http://www.nrcresearchpress.com/doi/pdf/10.1139/x95-012 |
id |
crcansciencepubl:10.1139/x95-012 |
---|---|
record_format |
openpolar |
spelling |
crcansciencepubl:10.1139/x95-012 2023-12-17T10:29:31+01:00 Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism Anamthawat-Jonsson, Kesara Heslop-Harrison, J.S. 1995 http://dx.doi.org/10.1139/x95-012 http://www.nrcresearchpress.com/doi/pdf/10.1139/x95-012 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Forest Research volume 25, issue 1, page 101-108 ISSN 0045-5067 1208-6037 Ecology Forestry Global and Planetary Change journal-article 1995 crcansciencepubl https://doi.org/10.1139/x95-012 2023-11-19T13:38:31Z The physical mapping of genes can reveal the organization of a genome and identify relationships of plant species, especially where they are involved in interspecific hybridization and polyploidy. Here we determine the chromosomal locations of the major ribosomal gene family (18S-5.8S-26S rDNA) by fluorescent in situ hybridization in two Icelandic birch species, Betulapubescens Ehrh. and Betulanana L. In the tetraploid birch (B. pubescens), the rDNA was localized on four major and two minor sites, while the diploid dwarf birch (B. nana) had four major sites. The major loci in both species were in nucleolus organizer regions, close to the centromeres of a pair of metacentric and a pair of sub-metacentric chromosomes. The dispersed interphase in situ hybridization pattern showed gene expression at all major sites. The two additional loci in B. pubescens, when detected, appeared to be sub-telomeric and inactive at interphase. Southern analysis of rDNA showed considerable restriction fragment length polymorphism in B. pubescens. Some polymorphism may reflect gene flow among populations and between the two co-existing birch species. The understanding of genome relationships, gene introgression, and evolution of birch species will be important to the breeding programmes steered towards environmental conservation and forestry. Article in Journal/Newspaper Dwarf birch Canadian Science Publishing (via Crossref) Canadian Journal of Forest Research 25 1 101 108 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Ecology Forestry Global and Planetary Change |
spellingShingle |
Ecology Forestry Global and Planetary Change Anamthawat-Jonsson, Kesara Heslop-Harrison, J.S. Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
topic_facet |
Ecology Forestry Global and Planetary Change |
description |
The physical mapping of genes can reveal the organization of a genome and identify relationships of plant species, especially where they are involved in interspecific hybridization and polyploidy. Here we determine the chromosomal locations of the major ribosomal gene family (18S-5.8S-26S rDNA) by fluorescent in situ hybridization in two Icelandic birch species, Betulapubescens Ehrh. and Betulanana L. In the tetraploid birch (B. pubescens), the rDNA was localized on four major and two minor sites, while the diploid dwarf birch (B. nana) had four major sites. The major loci in both species were in nucleolus organizer regions, close to the centromeres of a pair of metacentric and a pair of sub-metacentric chromosomes. The dispersed interphase in situ hybridization pattern showed gene expression at all major sites. The two additional loci in B. pubescens, when detected, appeared to be sub-telomeric and inactive at interphase. Southern analysis of rDNA showed considerable restriction fragment length polymorphism in B. pubescens. Some polymorphism may reflect gene flow among populations and between the two co-existing birch species. The understanding of genome relationships, gene introgression, and evolution of birch species will be important to the breeding programmes steered towards environmental conservation and forestry. |
format |
Article in Journal/Newspaper |
author |
Anamthawat-Jonsson, Kesara Heslop-Harrison, J.S. |
author_facet |
Anamthawat-Jonsson, Kesara Heslop-Harrison, J.S. |
author_sort |
Anamthawat-Jonsson, Kesara |
title |
Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
title_short |
Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
title_full |
Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
title_fullStr |
Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
title_full_unstemmed |
Molecular cytogenetics of Icelandic birch species: physical mapping by in situ hybridization and rDNA polymorphism |
title_sort |
molecular cytogenetics of icelandic birch species: physical mapping by in situ hybridization and rdna polymorphism |
publisher |
Canadian Science Publishing |
publishDate |
1995 |
url |
http://dx.doi.org/10.1139/x95-012 http://www.nrcresearchpress.com/doi/pdf/10.1139/x95-012 |
genre |
Dwarf birch |
genre_facet |
Dwarf birch |
op_source |
Canadian Journal of Forest Research volume 25, issue 1, page 101-108 ISSN 0045-5067 1208-6037 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/x95-012 |
container_title |
Canadian Journal of Forest Research |
container_volume |
25 |
container_issue |
1 |
container_start_page |
101 |
op_container_end_page |
108 |
_version_ |
1785581916117794816 |