Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen
The influence of environmental factors on the nitrogen fixation activity of free-living, terrestrial cyanobacteria from a high arctic area were investigated using experimental manipulations with two different types of field samples, including macroscopic sheets of Nostoc commune and soil samples wit...
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1999
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Online Access: | http://dx.doi.org/10.1139/w99-040 http://www.nrcresearchpress.com/doi/pdf/10.1139/w99-040 |
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crcansciencepubl:10.1139/w99-040 2024-06-23T07:49:53+00:00 Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen Liengen, Turid 1999 http://dx.doi.org/10.1139/w99-040 http://www.nrcresearchpress.com/doi/pdf/10.1139/w99-040 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 45, issue 7, page 573-581 ISSN 0008-4166 1480-3275 journal-article 1999 crcansciencepubl https://doi.org/10.1139/w99-040 2024-06-13T04:10:49Z The influence of environmental factors on the nitrogen fixation activity of free-living, terrestrial cyanobacteria from a high arctic area were investigated using experimental manipulations with two different types of field samples, including macroscopic sheets of Nostoc commune and soil samples with a cyanobacterial crust from a Puccinellia salt marsh. In addition, a cultured Anabaena sp. previously isolated from the salt marsh was examined. Nitrogen fixation activity was measured using the acetylene reduction method. The nitrogen fixation mainly took place in the light, but even after 12 h incubation in darkness, low activities were maintained. Phosphorus fertilization stimulated the nitrogen fixation activity, and the highest activities were obtained with about 300 μM phosphate, both in the field samples and the cultured Anabaena sp. Ammonium (28 mM) immediately inhibited the nitrogen fixation activity of the cultured Anabaena sp, whereas 14 mM urea and 540 μM glutamate led to a weaker and slower inhibition of the nitrogen fixation activity, showing that the cultured Anabaena sp. was able to assimilate these combined nitrogen sources. Nitrate did not have any inhibitory effect on nitrogen fixation activity, either in the field samples or in the cultured Anabaena sp. Both the field samples and the cultured Anabaena sp. showed tolerance against sodium chloride concentrations corresponding to the concentration in seawater. The temperature optimum of the nitrogen fixation activity of the cultured Anabaena sp. was about 20°C. Key words: nitrogen fixation, cyanobacteria, Nostoc commune, Anabaena sp., high arctic. Article in Journal/Newspaper Arctic Spitsbergen Canadian Science Publishing Arctic Canadian Journal of Microbiology 45 7 573 581 |
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Canadian Science Publishing |
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English |
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The influence of environmental factors on the nitrogen fixation activity of free-living, terrestrial cyanobacteria from a high arctic area were investigated using experimental manipulations with two different types of field samples, including macroscopic sheets of Nostoc commune and soil samples with a cyanobacterial crust from a Puccinellia salt marsh. In addition, a cultured Anabaena sp. previously isolated from the salt marsh was examined. Nitrogen fixation activity was measured using the acetylene reduction method. The nitrogen fixation mainly took place in the light, but even after 12 h incubation in darkness, low activities were maintained. Phosphorus fertilization stimulated the nitrogen fixation activity, and the highest activities were obtained with about 300 μM phosphate, both in the field samples and the cultured Anabaena sp. Ammonium (28 mM) immediately inhibited the nitrogen fixation activity of the cultured Anabaena sp, whereas 14 mM urea and 540 μM glutamate led to a weaker and slower inhibition of the nitrogen fixation activity, showing that the cultured Anabaena sp. was able to assimilate these combined nitrogen sources. Nitrate did not have any inhibitory effect on nitrogen fixation activity, either in the field samples or in the cultured Anabaena sp. Both the field samples and the cultured Anabaena sp. showed tolerance against sodium chloride concentrations corresponding to the concentration in seawater. The temperature optimum of the nitrogen fixation activity of the cultured Anabaena sp. was about 20°C. Key words: nitrogen fixation, cyanobacteria, Nostoc commune, Anabaena sp., high arctic. |
format |
Article in Journal/Newspaper |
author |
Liengen, Turid |
spellingShingle |
Liengen, Turid Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
author_facet |
Liengen, Turid |
author_sort |
Liengen, Turid |
title |
Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
title_short |
Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
title_full |
Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
title_fullStr |
Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
title_full_unstemmed |
Environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, Spitsbergen |
title_sort |
environmental factors influencing the nitrogen fixation activity of free-living terrestrial cyanobacteria from a high arctic area, spitsbergen |
publisher |
Canadian Science Publishing |
publishDate |
1999 |
url |
http://dx.doi.org/10.1139/w99-040 http://www.nrcresearchpress.com/doi/pdf/10.1139/w99-040 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
Canadian Journal of Microbiology volume 45, issue 7, page 573-581 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w99-040 |
container_title |
Canadian Journal of Microbiology |
container_volume |
45 |
container_issue |
7 |
container_start_page |
573 |
op_container_end_page |
581 |
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1802640580290805760 |