Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution
Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by...
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1998
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crcansciencepubl:10.1139/w98-107 2023-12-17T10:28:31+01:00 Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution Bahirathan, Mahesan Puente, Lawrence Seyfried, Patricia 1998 http://dx.doi.org/10.1139/w98-107 http://www.nrcresearchpress.com/doi/pdf/10.1139/w98-107 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 44, issue 11, page 1066-1071 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1998 crcansciencepubl https://doi.org/10.1139/w98-107 2023-11-19T13:38:24Z Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and other) depending on their origin. In three antibiotics, cephalothin, erythromycin, and vancomycin, there was a significant (p =< 0.05) association between susceptibility and source. Vancomycin resistance was significantly (p =< 0.001) higher in isolates from wild sources compared with that in isolates from other sources. The REA technique was performed on genomic DNA obtained from 17 Enterococcus mundtii isolates from: human (3), dog (4), horse (4), Canada goose (4), domestic goose (1), and Enterococcus mundtii ATCC 43186. A total of 12 different DNA types (A-L) were identified. Except for type D, 11 DNA types were unique and were distributed among dog (A, B, and C), human (E), horse (F, G, and H), Canada goose (I, J, and K), and domestic goose (L). Results suggested that vancomycin-susceptibility testing of yellow-pigmented enterococci may have potential value in the identification of sources of faecal pollution, especially when combined with traditional quantitative methods.Key words: yellow-pigmented enterococci, faecal pollution, antibiotic susceptibility, DNA typing. Article in Journal/Newspaper Canada Goose Canadian Science Publishing (via Crossref) Canada Canadian Journal of Microbiology 44 11 1066 1071 |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Bahirathan, Mahesan Puente, Lawrence Seyfried, Patricia Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and other) depending on their origin. In three antibiotics, cephalothin, erythromycin, and vancomycin, there was a significant (p =< 0.05) association between susceptibility and source. Vancomycin resistance was significantly (p =< 0.001) higher in isolates from wild sources compared with that in isolates from other sources. The REA technique was performed on genomic DNA obtained from 17 Enterococcus mundtii isolates from: human (3), dog (4), horse (4), Canada goose (4), domestic goose (1), and Enterococcus mundtii ATCC 43186. A total of 12 different DNA types (A-L) were identified. Except for type D, 11 DNA types were unique and were distributed among dog (A, B, and C), human (E), horse (F, G, and H), Canada goose (I, J, and K), and domestic goose (L). Results suggested that vancomycin-susceptibility testing of yellow-pigmented enterococci may have potential value in the identification of sources of faecal pollution, especially when combined with traditional quantitative methods.Key words: yellow-pigmented enterococci, faecal pollution, antibiotic susceptibility, DNA typing. |
format |
Article in Journal/Newspaper |
author |
Bahirathan, Mahesan Puente, Lawrence Seyfried, Patricia |
author_facet |
Bahirathan, Mahesan Puente, Lawrence Seyfried, Patricia |
author_sort |
Bahirathan, Mahesan |
title |
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
title_short |
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
title_full |
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
title_fullStr |
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
title_full_unstemmed |
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
title_sort |
use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution |
publisher |
Canadian Science Publishing |
publishDate |
1998 |
url |
http://dx.doi.org/10.1139/w98-107 http://www.nrcresearchpress.com/doi/pdf/10.1139/w98-107 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Canada Goose |
genre_facet |
Canada Goose |
op_source |
Canadian Journal of Microbiology volume 44, issue 11, page 1066-1071 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w98-107 |
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Canadian Journal of Microbiology |
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44 |
container_issue |
11 |
container_start_page |
1066 |
op_container_end_page |
1071 |
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1785580636024602624 |