Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae)
Four unique strains of Pseudallescheria boydii were isolated from oil-soaked soils in British Columbia and Alberta and compared to strains from cattle dung and raw sewage. Considerable variability in morphology, colony appearance, colony diameter, and temperature tolerance occurred among the strains...
Published in: | Canadian Journal of Microbiology |
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Canadian Science Publishing
1998
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Online Access: | http://dx.doi.org/10.1139/w97-152 http://www.nrcresearchpress.com/doi/pdf/10.1139/w97-152 |
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crcansciencepubl:10.1139/w97-152 2024-06-23T07:56:20+00:00 Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) April, T M Abbott, S P Foght, J M Currah, R S 1998 http://dx.doi.org/10.1139/w97-152 http://www.nrcresearchpress.com/doi/pdf/10.1139/w97-152 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 44, issue 3, page 270-278 ISSN 0008-4166 1480-3275 journal-article 1998 crcansciencepubl https://doi.org/10.1139/w97-152 2024-06-06T04:11:14Z Four unique strains of Pseudallescheria boydii were isolated from oil-soaked soils in British Columbia and Alberta and compared to strains from cattle dung and raw sewage. Considerable variability in morphology, colony appearance, colony diameter, and temperature tolerance occurred among the strains. They also varied in the sporogenous states produced in culture; all strains had a Scedosporium anamorph and either the Graphium anamorph or cleistothecial teleomorph. Conspecificity of the six isolates was inferred from their morphology and supported by restriction fragment length polymorphism profiles of the internally transcribed spacer region of rDNA and comparing these to Petriella sordida, a similar taxon in the Microascaceae. Three of the strains isolated from oil-contaminated soil and the strain from sewage were tested for their ability to utilize hydrocarbons by incubation with Prudhoe Bay Crude oil as the sole carbon source. Gas chromatographic analysis of the residual oil revealed that the strains isolated from oil-contaminated soil degraded the linear aliphatics. The strain from sewage, previously shown by others to utilize the volatile n-alkanes (i.e., ethane, propane, and butane), did not utilize the liquid saturate compounds. None of the strains was observed to degrade compounds in the aromatic fraction. Pseudallescheria boydii may be an important agent for in situ bioremediation of saturates in oil-contaminated sites.Key words: bioremediation, filamentous fungi, Graphium, hydrocarbon degradation, Scedosporium. Article in Journal/Newspaper Prudhoe Bay Canadian Science Publishing Canadian Journal of Microbiology 44 3 270 278 |
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English |
description |
Four unique strains of Pseudallescheria boydii were isolated from oil-soaked soils in British Columbia and Alberta and compared to strains from cattle dung and raw sewage. Considerable variability in morphology, colony appearance, colony diameter, and temperature tolerance occurred among the strains. They also varied in the sporogenous states produced in culture; all strains had a Scedosporium anamorph and either the Graphium anamorph or cleistothecial teleomorph. Conspecificity of the six isolates was inferred from their morphology and supported by restriction fragment length polymorphism profiles of the internally transcribed spacer region of rDNA and comparing these to Petriella sordida, a similar taxon in the Microascaceae. Three of the strains isolated from oil-contaminated soil and the strain from sewage were tested for their ability to utilize hydrocarbons by incubation with Prudhoe Bay Crude oil as the sole carbon source. Gas chromatographic analysis of the residual oil revealed that the strains isolated from oil-contaminated soil degraded the linear aliphatics. The strain from sewage, previously shown by others to utilize the volatile n-alkanes (i.e., ethane, propane, and butane), did not utilize the liquid saturate compounds. None of the strains was observed to degrade compounds in the aromatic fraction. Pseudallescheria boydii may be an important agent for in situ bioremediation of saturates in oil-contaminated sites.Key words: bioremediation, filamentous fungi, Graphium, hydrocarbon degradation, Scedosporium. |
format |
Article in Journal/Newspaper |
author |
April, T M Abbott, S P Foght, J M Currah, R S |
spellingShingle |
April, T M Abbott, S P Foght, J M Currah, R S Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
author_facet |
April, T M Abbott, S P Foght, J M Currah, R S |
author_sort |
April, T M |
title |
Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
title_short |
Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
title_full |
Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
title_fullStr |
Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
title_full_unstemmed |
Degradation of hydrocarbons in crude oil by the ascomycete Pseudallescheria boydii(Microascaceae) |
title_sort |
degradation of hydrocarbons in crude oil by the ascomycete pseudallescheria boydii(microascaceae) |
publisher |
Canadian Science Publishing |
publishDate |
1998 |
url |
http://dx.doi.org/10.1139/w97-152 http://www.nrcresearchpress.com/doi/pdf/10.1139/w97-152 |
genre |
Prudhoe Bay |
genre_facet |
Prudhoe Bay |
op_source |
Canadian Journal of Microbiology volume 44, issue 3, page 270-278 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w97-152 |
container_title |
Canadian Journal of Microbiology |
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44 |
container_issue |
3 |
container_start_page |
270 |
op_container_end_page |
278 |
_version_ |
1802649372209446912 |