Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic
The abundance and structure of archaeal and bacterial communities from the active layer and the associated permafrost of a moderately acidic (pH < 5.0) High Arctic wetland (Axel Heiberg Island, Nunavut, Canada) were investigated using culture- and molecular-based methods. Aerobic viable cell coun...
Published in: | Canadian Journal of Microbiology |
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crcansciencepubl:10.1139/w11-004 2024-09-15T17:56:52+00:00 Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic Wilhelm, Roland C. Niederberger, Thomas D. Greer, Charles Whyte, Lyle G. 2011 http://dx.doi.org/10.1139/w11-004 http://www.nrcresearchpress.com/doi/full-xml/10.1139/w11-004 http://www.nrcresearchpress.com/doi/pdf/10.1139/w11-004 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 57, issue 4, page 303-315 ISSN 0008-4166 1480-3275 journal-article 2011 crcansciencepubl https://doi.org/10.1139/w11-004 2024-09-05T04:11:14Z The abundance and structure of archaeal and bacterial communities from the active layer and the associated permafrost of a moderately acidic (pH < 5.0) High Arctic wetland (Axel Heiberg Island, Nunavut, Canada) were investigated using culture- and molecular-based methods. Aerobic viable cell counts from the active layer were ∼100-fold greater than those from the permafrost (2.5 × 10 5 CFU·(g soil dry mass) –1 ); however, a greater diversity of isolates were cultured from permafrost, as determined by 16S rRNA gene sequencing. Isolates from both layers demonstrated growth characteristics of a psychrotolerant, halotolerant, and acidotolerant community. Archaea constituted 0.1% of the total 16S rRNA gene copy number and, in the 16S rRNA gene clone library, predominantly (71% and 95%) consisted of Crenarchaeota related to Group I. 1b. In contrast, bacterial communities were diverse (Shannon’s diversity index, H = ∼4), with Acidobacteria constituting the largest division of active layer clones (30%) and Actinobacteria most abundant in permafrost (28%). Direct comparisons of 16S rRNA gene sequence data highlighted significant differences between the bacterial communities of each layer, with the greatest differences occurring within Actinobacteria. Comparisons of 16S rRNA gene sequences with those from other Arctic permafrost and cold-temperature wetlands revealed commonly occurring taxa within the phyla Chloroflexi, Acidobacteria, and Actinobacteria (families Intrasporangiaceae and Rubrobacteraceae). Article in Journal/Newspaper Axel Heiberg Island Nunavut permafrost Canadian Science Publishing Canadian Journal of Microbiology 57 4 303 315 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
The abundance and structure of archaeal and bacterial communities from the active layer and the associated permafrost of a moderately acidic (pH < 5.0) High Arctic wetland (Axel Heiberg Island, Nunavut, Canada) were investigated using culture- and molecular-based methods. Aerobic viable cell counts from the active layer were ∼100-fold greater than those from the permafrost (2.5 × 10 5 CFU·(g soil dry mass) –1 ); however, a greater diversity of isolates were cultured from permafrost, as determined by 16S rRNA gene sequencing. Isolates from both layers demonstrated growth characteristics of a psychrotolerant, halotolerant, and acidotolerant community. Archaea constituted 0.1% of the total 16S rRNA gene copy number and, in the 16S rRNA gene clone library, predominantly (71% and 95%) consisted of Crenarchaeota related to Group I. 1b. In contrast, bacterial communities were diverse (Shannon’s diversity index, H = ∼4), with Acidobacteria constituting the largest division of active layer clones (30%) and Actinobacteria most abundant in permafrost (28%). Direct comparisons of 16S rRNA gene sequence data highlighted significant differences between the bacterial communities of each layer, with the greatest differences occurring within Actinobacteria. Comparisons of 16S rRNA gene sequences with those from other Arctic permafrost and cold-temperature wetlands revealed commonly occurring taxa within the phyla Chloroflexi, Acidobacteria, and Actinobacteria (families Intrasporangiaceae and Rubrobacteraceae). |
format |
Article in Journal/Newspaper |
author |
Wilhelm, Roland C. Niederberger, Thomas D. Greer, Charles Whyte, Lyle G. |
spellingShingle |
Wilhelm, Roland C. Niederberger, Thomas D. Greer, Charles Whyte, Lyle G. Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
author_facet |
Wilhelm, Roland C. Niederberger, Thomas D. Greer, Charles Whyte, Lyle G. |
author_sort |
Wilhelm, Roland C. |
title |
Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
title_short |
Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
title_full |
Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
title_fullStr |
Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
title_full_unstemmed |
Microbial diversity of active layer and permafrost in an acidic wetland from the Canadian High Arctic |
title_sort |
microbial diversity of active layer and permafrost in an acidic wetland from the canadian high arctic |
publisher |
Canadian Science Publishing |
publishDate |
2011 |
url |
http://dx.doi.org/10.1139/w11-004 http://www.nrcresearchpress.com/doi/full-xml/10.1139/w11-004 http://www.nrcresearchpress.com/doi/pdf/10.1139/w11-004 |
genre |
Axel Heiberg Island Nunavut permafrost |
genre_facet |
Axel Heiberg Island Nunavut permafrost |
op_source |
Canadian Journal of Microbiology volume 57, issue 4, page 303-315 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w11-004 |
container_title |
Canadian Journal of Microbiology |
container_volume |
57 |
container_issue |
4 |
container_start_page |
303 |
op_container_end_page |
315 |
_version_ |
1810433040551247872 |