The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology.
The marine Antarctic ciliate, Euplotes nobilii , secretes a family of water-borne signal proteins, denoted as pheromones, which control vegetative proliferation and mating in the cell. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernata...
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2009
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crcansciencepubl:10.1139/w08-122 2024-06-23T07:47:47+00:00 The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. La Terza, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo 2009 http://dx.doi.org/10.1139/w08-122 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W08-122 http://www.nrcresearchpress.com/doi/pdf/10.1139/W08-122 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 55, issue 1, page 57-62 ISSN 0008-4166 1480-3275 journal-article 2009 crcansciencepubl https://doi.org/10.1139/w08-122 2024-06-06T04:11:18Z The marine Antarctic ciliate, Euplotes nobilii , secretes a family of water-borne signal proteins, denoted as pheromones, which control vegetative proliferation and mating in the cell. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernatant of wild-type strains, we designed probes to identify their encoding genes in the cell somatic nucleus (macronucleus). The full-length gene of the pheromone En-6 was determined and found to contain an open-reading frame specific for the synthesis of the En-6 cytoplasmic precursor (pre-pro-En-6), which requires 2 proteolytic cleavages to remove the signal peptide (pre) and the prosegment before secretion of the mature protein. In contrast to the sequence variability that distinguishes the secreted pheromones, the pre- and pro-sequences appear to be tightly conserved and useful for the construction of probes to clone every other E. nobilii pheromone gene. Potential intron sequences in the coding region of the En-6 gene imply the synthesis of more En-6 isoforms. Article in Journal/Newspaper Antarc* Antarctic Canadian Science Publishing Antarctic The Antarctic Canadian Journal of Microbiology 55 1 57 62 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
The marine Antarctic ciliate, Euplotes nobilii , secretes a family of water-borne signal proteins, denoted as pheromones, which control vegetative proliferation and mating in the cell. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernatant of wild-type strains, we designed probes to identify their encoding genes in the cell somatic nucleus (macronucleus). The full-length gene of the pheromone En-6 was determined and found to contain an open-reading frame specific for the synthesis of the En-6 cytoplasmic precursor (pre-pro-En-6), which requires 2 proteolytic cleavages to remove the signal peptide (pre) and the prosegment before secretion of the mature protein. In contrast to the sequence variability that distinguishes the secreted pheromones, the pre- and pro-sequences appear to be tightly conserved and useful for the construction of probes to clone every other E. nobilii pheromone gene. Potential intron sequences in the coding region of the En-6 gene imply the synthesis of more En-6 isoforms. |
format |
Article in Journal/Newspaper |
author |
La Terza, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo |
spellingShingle |
La Terza, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
author_facet |
La Terza, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo |
author_sort |
La Terza, Antonietta |
title |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_short |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_full |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_fullStr |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_full_unstemmed |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan Euplotes nobilii: structure of the gene coding for the En-6 pheromoneThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_sort |
water-borne protein signals (pheromones) of the antarctic ciliated protozoan euplotes nobilii: structure of the gene coding for the en-6 pheromonethis article is one of a selection of papers in the special issue on polar and alpine microbiology. |
publisher |
Canadian Science Publishing |
publishDate |
2009 |
url |
http://dx.doi.org/10.1139/w08-122 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W08-122 http://www.nrcresearchpress.com/doi/pdf/10.1139/W08-122 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Canadian Journal of Microbiology volume 55, issue 1, page 57-62 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w08-122 |
container_title |
Canadian Journal of Microbiology |
container_volume |
55 |
container_issue |
1 |
container_start_page |
57 |
op_container_end_page |
62 |
_version_ |
1802637930228875264 |