Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology.
Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic m...
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crcansciencepubl:10.1139/w08-119 2024-06-23T07:46:30+00:00 Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. Miller, Robert V. Gammon, Katharine Day, Martin J. 2009 http://dx.doi.org/10.1139/w08-119 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W08-119 http://www.nrcresearchpress.com/doi/pdf/10.1139/W08-119 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 55, issue 1, page 37-45 ISSN 0008-4166 1480-3275 journal-article 2009 crcansciencepubl https://doi.org/10.1139/w08-119 2024-05-30T08:13:47Z Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic marine waters and a penguin rookery, for 2 reasons: (i) as a measure of human impact and (ii) as a potential “snapshot” of the preantibiotic world. Samples were taken at 4 established sampling sites near Palmer Station, which is situated at the southern end of the Palmer Archipelago (64°10′S, 61°50′W). Sites were chosen to provide different potentials for human contamination. Forty 50 mL samples of seawater were collected and colony-forming units (CFU)/mL were determined at 6 and 20 °C. For this study, presumed psychrophiles (growth at 6 °C) were assumed to be native to Antarctic waters, whereas presumed mesophiles (growth at 20 °C but not at 6 °C) were taken to represent introduced organisms. The 20–6 °C CFU/mL ratio was used as a measure of the relative impact to the ecosystem of presumably introduced organisms. This ratio was highest at the site nearest to Palmer Station and decreased with distance from it, suggesting that human presence has impacted the natural microbial flora of the site. The frequency of resistance to 5 common antibiotics was determined in each group of isolates. Overall drug resistance was higher among the presumed mesophiles than the presumed psychrophiles and increased with proximity to Palmer Station, with the presumed mesophiles showing higher frequencies of single and multiple drug resistance than the psychrophile population. The frequency of multidrug resistance followed the same pattern. It appears that multidrug resistance is low among native Antarctic bacteria but is increased by human habitation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Palmer Archipelago Canadian Science Publishing Antarctic The Antarctic Palmer Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer-Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer Archipelago ENVELOPE(-62.833,-62.833,-64.250,-64.250) Canadian Journal of Microbiology 55 1 37 45 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
Antibiotic resistance in aquatic bacteria has increased steadily as a consequence of the widespread use of antibiotics, but practice and international treaty should have limited antibiotic contamination in Antarctica. We estimated antibiotic resistance in microorganisms isolated from the Antarctic marine waters and a penguin rookery, for 2 reasons: (i) as a measure of human impact and (ii) as a potential “snapshot” of the preantibiotic world. Samples were taken at 4 established sampling sites near Palmer Station, which is situated at the southern end of the Palmer Archipelago (64°10′S, 61°50′W). Sites were chosen to provide different potentials for human contamination. Forty 50 mL samples of seawater were collected and colony-forming units (CFU)/mL were determined at 6 and 20 °C. For this study, presumed psychrophiles (growth at 6 °C) were assumed to be native to Antarctic waters, whereas presumed mesophiles (growth at 20 °C but not at 6 °C) were taken to represent introduced organisms. The 20–6 °C CFU/mL ratio was used as a measure of the relative impact to the ecosystem of presumably introduced organisms. This ratio was highest at the site nearest to Palmer Station and decreased with distance from it, suggesting that human presence has impacted the natural microbial flora of the site. The frequency of resistance to 5 common antibiotics was determined in each group of isolates. Overall drug resistance was higher among the presumed mesophiles than the presumed psychrophiles and increased with proximity to Palmer Station, with the presumed mesophiles showing higher frequencies of single and multiple drug resistance than the psychrophile population. The frequency of multidrug resistance followed the same pattern. It appears that multidrug resistance is low among native Antarctic bacteria but is increased by human habitation. |
format |
Article in Journal/Newspaper |
author |
Miller, Robert V. Gammon, Katharine Day, Martin J. |
spellingShingle |
Miller, Robert V. Gammon, Katharine Day, Martin J. Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
author_facet |
Miller, Robert V. Gammon, Katharine Day, Martin J. |
author_sort |
Miller, Robert V. |
title |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_short |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_full |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_fullStr |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_full_unstemmed |
Antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near Palmer Station, AntarcticaThis article is one of a selection of papers in the Special Issue on Polar and Alpine Microbiology. |
title_sort |
antibiotic resistance among bacteria isolated from seawater and penguin fecal samples collected near palmer station, antarcticathis article is one of a selection of papers in the special issue on polar and alpine microbiology. |
publisher |
Canadian Science Publishing |
publishDate |
2009 |
url |
http://dx.doi.org/10.1139/w08-119 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W08-119 http://www.nrcresearchpress.com/doi/pdf/10.1139/W08-119 |
long_lat |
ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-62.833,-62.833,-64.250,-64.250) |
geographic |
Antarctic The Antarctic Palmer Station Palmer-Station Palmer Archipelago |
geographic_facet |
Antarctic The Antarctic Palmer Station Palmer-Station Palmer Archipelago |
genre |
Antarc* Antarctic Antarctica Palmer Archipelago |
genre_facet |
Antarc* Antarctic Antarctica Palmer Archipelago |
op_source |
Canadian Journal of Microbiology volume 55, issue 1, page 37-45 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w08-119 |
container_title |
Canadian Journal of Microbiology |
container_volume |
55 |
container_issue |
1 |
container_start_page |
37 |
op_container_end_page |
45 |
_version_ |
1802646377355804672 |