Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region
The Qinghai-Tibet Plateau in east Asia is a unique and important permafrost environment. However, its microbiology remains largely unexplored to date. In this study, sediment samples were collected from the Qinghai-Tibet Plateau permafrost region, bacteria isolation procedures were performed 8 times...
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crcansciencepubl:10.1139/w07-031 2024-09-15T18:29:40+00:00 Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region Zhang, Gaosen Niu, Fujun Ma, Xiaojun Liu, Wei Dong, Maoxing Feng, Huyuan An, Lizhe Cheng, Guodong 2007 http://dx.doi.org/10.1139/w07-031 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W07-031 http://www.nrcresearchpress.com/doi/pdf/10.1139/W07-031 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 53, issue 8, page 1000-1010 ISSN 0008-4166 1480-3275 journal-article 2007 crcansciencepubl https://doi.org/10.1139/w07-031 2024-07-18T04:13:37Z The Qinghai-Tibet Plateau in east Asia is a unique and important permafrost environment. However, its microbiology remains largely unexplored to date. In this study, sediment samples were collected from the Qinghai-Tibet Plateau permafrost region, bacteria isolation procedures were performed 8 times, and the samples incubated at 4 °C for nearly 3 months. The number of colony forming units (cfu) ranged from 0 to 10 7 /(g dry soil). The quantity of culturable bacteria grew exponentially within the first few weeks, and then slowed gradually to a plateau. Phylogenetic analyses indicated that all the isolates fell into 6 categories: high G+C Gram-positive bacteria, low G+C Gram-positive bacteria, α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, and Cytophaga–Flavobacterium–Bacteroides group bacteria. The isolates belong to 19 genera, but the genera Arthrobacter and Pseudomonas were predominant. With the increase in incubation time, the isolated populations changed in terms of both species and their respective quantities. Of the 33 analyzed isolates, 9 isolates related to 8 genera might be new taxa. These results suggest that the Qinghai-Tibet Plateau permafrost region is a specific ecologic niche that accommodates an original microbial assemblage. Article in Journal/Newspaper permafrost Canadian Science Publishing Canadian Journal of Microbiology 53 8 1000 1010 |
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Canadian Science Publishing |
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crcansciencepubl |
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English |
description |
The Qinghai-Tibet Plateau in east Asia is a unique and important permafrost environment. However, its microbiology remains largely unexplored to date. In this study, sediment samples were collected from the Qinghai-Tibet Plateau permafrost region, bacteria isolation procedures were performed 8 times, and the samples incubated at 4 °C for nearly 3 months. The number of colony forming units (cfu) ranged from 0 to 10 7 /(g dry soil). The quantity of culturable bacteria grew exponentially within the first few weeks, and then slowed gradually to a plateau. Phylogenetic analyses indicated that all the isolates fell into 6 categories: high G+C Gram-positive bacteria, low G+C Gram-positive bacteria, α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, and Cytophaga–Flavobacterium–Bacteroides group bacteria. The isolates belong to 19 genera, but the genera Arthrobacter and Pseudomonas were predominant. With the increase in incubation time, the isolated populations changed in terms of both species and their respective quantities. Of the 33 analyzed isolates, 9 isolates related to 8 genera might be new taxa. These results suggest that the Qinghai-Tibet Plateau permafrost region is a specific ecologic niche that accommodates an original microbial assemblage. |
format |
Article in Journal/Newspaper |
author |
Zhang, Gaosen Niu, Fujun Ma, Xiaojun Liu, Wei Dong, Maoxing Feng, Huyuan An, Lizhe Cheng, Guodong |
spellingShingle |
Zhang, Gaosen Niu, Fujun Ma, Xiaojun Liu, Wei Dong, Maoxing Feng, Huyuan An, Lizhe Cheng, Guodong Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
author_facet |
Zhang, Gaosen Niu, Fujun Ma, Xiaojun Liu, Wei Dong, Maoxing Feng, Huyuan An, Lizhe Cheng, Guodong |
author_sort |
Zhang, Gaosen |
title |
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
title_short |
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
title_full |
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
title_fullStr |
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
title_full_unstemmed |
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region |
title_sort |
phylogenetic diversity of bacteria isolates from the qinghai-tibet plateau permafrost region |
publisher |
Canadian Science Publishing |
publishDate |
2007 |
url |
http://dx.doi.org/10.1139/w07-031 http://www.nrcresearchpress.com/doi/full-xml/10.1139/W07-031 http://www.nrcresearchpress.com/doi/pdf/10.1139/W07-031 |
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permafrost |
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permafrost |
op_source |
Canadian Journal of Microbiology volume 53, issue 8, page 1000-1010 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w07-031 |
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Canadian Journal of Microbiology |
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53 |
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8 |
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1000 |
op_container_end_page |
1010 |
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1810471082806738944 |