Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA
Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (α, β, ε, and ι) for classif...
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crcansciencepubl:10.1139/w02-015 2024-09-15T17:55:25+00:00 Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA Aschfalk, A Müller, W 2002 http://dx.doi.org/10.1139/w02-015 http://www.nrcresearchpress.com/doi/pdf/10.1139/w02-015 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 48, issue 4, page 365-368 ISSN 0008-4166 1480-3275 journal-article 2002 crcansciencepubl https://doi.org/10.1139/w02-015 2024-07-18T04:13:37Z Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (α, β, ε, and ι) for classification into toxin types and for genes encoding enterotoxin and the novel β2 toxin for further subclassification. In addition, a commercial enzyme-linked immunosorbent assay (ELISA) kit for detection of C. perfringens α, β, and ε toxin was used. Clostridium perfringens could be isolated in 37 fecal samples (38.9%) from cod. All isolates were C. perfringens toxin type A (α toxin positive) as determined by PCR and also ELISA. In addition, in isolates from two cod (2.1%) the gene encoding for β2 toxin was found (A, β2) by PCR. Genes encoding for β, ε, and ι toxins and enterotoxin were not found. This is the first detection of C. perfringens alpha and β2 toxin in cod and of β2 toxin in fish in general. The origin of this bacterium in cod is discussed.Key words: Clostridium perfringens, cod, Gadus morhua L. Article in Journal/Newspaper atlantic cod Gadus morhua Canadian Science Publishing Canadian Journal of Microbiology 48 4 365 368 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
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English |
description |
Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (α, β, ε, and ι) for classification into toxin types and for genes encoding enterotoxin and the novel β2 toxin for further subclassification. In addition, a commercial enzyme-linked immunosorbent assay (ELISA) kit for detection of C. perfringens α, β, and ε toxin was used. Clostridium perfringens could be isolated in 37 fecal samples (38.9%) from cod. All isolates were C. perfringens toxin type A (α toxin positive) as determined by PCR and also ELISA. In addition, in isolates from two cod (2.1%) the gene encoding for β2 toxin was found (A, β2) by PCR. Genes encoding for β, ε, and ι toxins and enterotoxin were not found. This is the first detection of C. perfringens alpha and β2 toxin in cod and of β2 toxin in fish in general. The origin of this bacterium in cod is discussed.Key words: Clostridium perfringens, cod, Gadus morhua L. |
format |
Article in Journal/Newspaper |
author |
Aschfalk, A Müller, W |
spellingShingle |
Aschfalk, A Müller, W Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
author_facet |
Aschfalk, A Müller, W |
author_sort |
Aschfalk, A |
title |
Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
title_short |
Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
title_full |
Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
title_fullStr |
Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
title_full_unstemmed |
Clostridium perfringenstoxin types from wild-caught Atlantic cod ( Gadus morhua L.), determined by PCR and ELISA |
title_sort |
clostridium perfringenstoxin types from wild-caught atlantic cod ( gadus morhua l.), determined by pcr and elisa |
publisher |
Canadian Science Publishing |
publishDate |
2002 |
url |
http://dx.doi.org/10.1139/w02-015 http://www.nrcresearchpress.com/doi/pdf/10.1139/w02-015 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Canadian Journal of Microbiology volume 48, issue 4, page 365-368 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/w02-015 |
container_title |
Canadian Journal of Microbiology |
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48 |
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4 |
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365 |
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368 |
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1810431709141794816 |