On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5)
pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoichiometric formation of ammonia, carbonic acid, and hydrazine, and having a k cat of about [Formula: see...
| Published in: | Canadian Journal of Biochemistry |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
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Canadian Science Publishing
1973
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/o73-174 http://www.nrcresearchpress.com/doi/pdf/10.1139/o73-174 |
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crcansciencepubl:10.1139/o73-174 |
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crcansciencepubl:10.1139/o73-174 2024-05-12T08:02:20+00:00 On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) Gazzola, Carlo Blakeley, Robert L. Zerner, Burt 1973 http://dx.doi.org/10.1139/o73-174 http://www.nrcresearchpress.com/doi/pdf/10.1139/o73-174 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Biochemistry volume 51, issue 9, page 1325-1330 ISSN 0008-4018 General Medicine journal-article 1973 crcansciencepubl https://doi.org/10.1139/o73-174 2024-04-18T06:54:48Z pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoichiometric formation of ammonia, carbonic acid, and hydrazine, and having a k cat of about [Formula: see text] that of urea at pH 7. Article in Journal/Newspaper Carbonic acid Canadian Science Publishing Canadian Journal of Biochemistry 51 9 1325 1330 |
| institution |
Open Polar |
| collection |
Canadian Science Publishing |
| op_collection_id |
crcansciencepubl |
| language |
English |
| topic |
General Medicine |
| spellingShingle |
General Medicine Gazzola, Carlo Blakeley, Robert L. Zerner, Burt On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| topic_facet |
General Medicine |
| description |
pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoichiometric formation of ammonia, carbonic acid, and hydrazine, and having a k cat of about [Formula: see text] that of urea at pH 7. |
| format |
Article in Journal/Newspaper |
| author |
Gazzola, Carlo Blakeley, Robert L. Zerner, Burt |
| author_facet |
Gazzola, Carlo Blakeley, Robert L. Zerner, Burt |
| author_sort |
Gazzola, Carlo |
| title |
On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| title_short |
On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| title_full |
On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| title_fullStr |
On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| title_full_unstemmed |
On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5) |
| title_sort |
on the substrate specificity of jack bean urease (urea amidohydrolase, ec 3.5.1.5) |
| publisher |
Canadian Science Publishing |
| publishDate |
1973 |
| url |
http://dx.doi.org/10.1139/o73-174 http://www.nrcresearchpress.com/doi/pdf/10.1139/o73-174 |
| genre |
Carbonic acid |
| genre_facet |
Carbonic acid |
| op_source |
Canadian Journal of Biochemistry volume 51, issue 9, page 1325-1330 ISSN 0008-4018 |
| op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
| op_doi |
https://doi.org/10.1139/o73-174 |
| container_title |
Canadian Journal of Biochemistry |
| container_volume |
51 |
| container_issue |
9 |
| container_start_page |
1325 |
| op_container_end_page |
1330 |
| _version_ |
1798844422592397312 |