On the Substrate Specificity of Jack Bean Urease (Urea amidohydrolase, EC 3.5.1.5)

pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoichiometric formation of ammonia, carbonic acid, and hydrazine, and having a k cat of about [Formula: see...

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Bibliographic Details
Published in:Canadian Journal of Biochemistry
Main Authors: Gazzola, Carlo, Blakeley, Robert L., Zerner, Burt
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1973
Subjects:
General Medicine
Carbonic acid
Online Access:http://dx.doi.org/10.1139/o73-174
http://www.nrcresearchpress.com/doi/pdf/10.1139/o73-174
Description
Summary:pH-Stat studies prove that N-methylurea, ethyl carbamate, and carbamoyl azide are not substrates for jack bean urease. Semicarbazide is a good substrate, showing Michaelis–Menten kinetics and stoichiometric formation of ammonia, carbonic acid, and hydrazine, and having a k cat of about [Formula: see text] that of urea at pH 7.

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