Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium
Sixty-three bacterial isolates from antarctic sandstone samples (Linnaeus Terrace, Asgard Range, McMurdo Dry Valleys) were screened for the presence of plasmid DNA. Twenty-seven percent of all the isolates (mainly Gram-positives) harbored one or more plasmids of low molecular mass (1.1–16.3 kb). Str...
Published in: | Canadian Journal of Microbiology |
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1996
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crcansciencepubl:10.1139/m96-077 2023-12-17T10:20:48+01:00 Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium Gliesche, Christian G. Jendrach, Marina Peissl, Klaus Siebert, Jörg Hirsch, Peter 1996 http://dx.doi.org/10.1139/m96-077 http://www.nrcresearchpress.com/doi/pdf/10.1139/m96-077 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 42, issue 6, page 571-576 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1996 crcansciencepubl https://doi.org/10.1139/m96-077 2023-11-19T13:38:15Z Sixty-three bacterial isolates from antarctic sandstone samples (Linnaeus Terrace, Asgard Range, McMurdo Dry Valleys) were screened for the presence of plasmid DNA. Twenty-seven percent of all the isolates (mainly Gram-positives) harbored one or more plasmids of low molecular mass (1.1–16.3 kb). Strain AA-341 contained plasmid pAA-1 (2.9 kb), as demonstrated by agarose gel electrophoresis and restriction endonuclease digests. This plasmid conferred resistance to chromium and ampicillin. It was not conjugative, but it could be transferred by electroporation to chromium- and ampicillin-sensitive strains AA-330, AA-338, and E. coli HB101. A restriction map of pAA-1 was constructed with HindIII, SalI, ScaI, AvaII, EcoRI, PvuII, BamHI, and DraI. Electrotransfer of this plasmid from E. coli HB101(pAA-1) to strain AA-330 was demonstrated. By natural genetic transformation, plasmid pAA-1 could be transferred into the sensitive strain AA-334, which thereby became resistant to chromium and ampicillin. The importance of such processes for the colonization of stressed environments is discussed.Key words: Antarctica, cryptoendolithic bacteria, plasmids, resistance to chromium, natural transformation. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Canadian Science Publishing (via Crossref) Antarctic McMurdo Dry Valleys Paa ENVELOPE(-53.483,-53.483,66.017,66.017) Linnaeus Terrace ENVELOPE(161.083,161.083,-77.600,-77.600) Asgard Range ENVELOPE(161.500,161.500,-77.617,-77.617) Canadian Journal of Microbiology 42 6 571 576 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Gliesche, Christian G. Jendrach, Marina Peissl, Klaus Siebert, Jörg Hirsch, Peter Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Sixty-three bacterial isolates from antarctic sandstone samples (Linnaeus Terrace, Asgard Range, McMurdo Dry Valleys) were screened for the presence of plasmid DNA. Twenty-seven percent of all the isolates (mainly Gram-positives) harbored one or more plasmids of low molecular mass (1.1–16.3 kb). Strain AA-341 contained plasmid pAA-1 (2.9 kb), as demonstrated by agarose gel electrophoresis and restriction endonuclease digests. This plasmid conferred resistance to chromium and ampicillin. It was not conjugative, but it could be transferred by electroporation to chromium- and ampicillin-sensitive strains AA-330, AA-338, and E. coli HB101. A restriction map of pAA-1 was constructed with HindIII, SalI, ScaI, AvaII, EcoRI, PvuII, BamHI, and DraI. Electrotransfer of this plasmid from E. coli HB101(pAA-1) to strain AA-330 was demonstrated. By natural genetic transformation, plasmid pAA-1 could be transferred into the sensitive strain AA-334, which thereby became resistant to chromium and ampicillin. The importance of such processes for the colonization of stressed environments is discussed.Key words: Antarctica, cryptoendolithic bacteria, plasmids, resistance to chromium, natural transformation. |
format |
Article in Journal/Newspaper |
author |
Gliesche, Christian G. Jendrach, Marina Peissl, Klaus Siebert, Jörg Hirsch, Peter |
author_facet |
Gliesche, Christian G. Jendrach, Marina Peissl, Klaus Siebert, Jörg Hirsch, Peter |
author_sort |
Gliesche, Christian G. |
title |
Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
title_short |
Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
title_full |
Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
title_fullStr |
Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
title_full_unstemmed |
Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium |
title_sort |
characterization and transformation of plasmid paa-1 found in an antarctic cryptoendolithic bacterium |
publisher |
Canadian Science Publishing |
publishDate |
1996 |
url |
http://dx.doi.org/10.1139/m96-077 http://www.nrcresearchpress.com/doi/pdf/10.1139/m96-077 |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) ENVELOPE(161.083,161.083,-77.600,-77.600) ENVELOPE(161.500,161.500,-77.617,-77.617) |
geographic |
Antarctic McMurdo Dry Valleys Paa Linnaeus Terrace Asgard Range |
geographic_facet |
Antarctic McMurdo Dry Valleys Paa Linnaeus Terrace Asgard Range |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_source |
Canadian Journal of Microbiology volume 42, issue 6, page 571-576 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m96-077 |
container_title |
Canadian Journal of Microbiology |
container_volume |
42 |
container_issue |
6 |
container_start_page |
571 |
op_container_end_page |
576 |
_version_ |
1785526284190744576 |