Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2
The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was originally isolated from the rhizosphere of plants growing in the Canadian High Arctic. Here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 °C, a temperature at w...
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1995
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crcansciencepubl:10.1139/m95-107 2024-09-09T19:25:38+00:00 Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 Sun, Xiuying Griffith, Marilyn Pasternak, J. J. Glick, Bernard R. 1995 http://dx.doi.org/10.1139/m95-107 http://www.nrcresearchpress.com/doi/pdf/10.1139/m95-107 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 41, issue 9, page 776-784 ISSN 0008-4166 1480-3275 journal-article 1995 crcansciencepubl https://doi.org/10.1139/m95-107 2024-08-01T04:10:02Z The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was originally isolated from the rhizosphere of plants growing in the Canadian High Arctic. Here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 °C, a temperature at which only a relatively small number of bacteria are able to proliferate and function. In addition, the bacterium survived exposure to freezing temperatures, i.e., −20 and −50 °C. In an effort to determine the mechanistic basis for this behaviour, it was discovered that following growth at 5 °C, P. putida GR12-2 synthesized and secreted to the growth medium a protein with antifreeze activity. Analysis of the spent growth medium, following concentration by ultrafiltration, by SDS-polyacrylamide gel electrophoresis revealed the presence of one major protein with a molecular mass of approximately 32–34 kDa and a number of minor proteins. However, at this point it is not known which of these proteins contains the antifreeze activity.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, antifreeze protein. Article in Journal/Newspaper Arctic Canadian Science Publishing Arctic Canadian Journal of Microbiology 41 9 776 784 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was originally isolated from the rhizosphere of plants growing in the Canadian High Arctic. Here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 °C, a temperature at which only a relatively small number of bacteria are able to proliferate and function. In addition, the bacterium survived exposure to freezing temperatures, i.e., −20 and −50 °C. In an effort to determine the mechanistic basis for this behaviour, it was discovered that following growth at 5 °C, P. putida GR12-2 synthesized and secreted to the growth medium a protein with antifreeze activity. Analysis of the spent growth medium, following concentration by ultrafiltration, by SDS-polyacrylamide gel electrophoresis revealed the presence of one major protein with a molecular mass of approximately 32–34 kDa and a number of minor proteins. However, at this point it is not known which of these proteins contains the antifreeze activity.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, antifreeze protein. |
format |
Article in Journal/Newspaper |
author |
Sun, Xiuying Griffith, Marilyn Pasternak, J. J. Glick, Bernard R. |
spellingShingle |
Sun, Xiuying Griffith, Marilyn Pasternak, J. J. Glick, Bernard R. Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
author_facet |
Sun, Xiuying Griffith, Marilyn Pasternak, J. J. Glick, Bernard R. |
author_sort |
Sun, Xiuying |
title |
Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
title_short |
Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
title_full |
Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
title_fullStr |
Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
title_full_unstemmed |
Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 |
title_sort |
low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium pseudomonas putida gr12-2 |
publisher |
Canadian Science Publishing |
publishDate |
1995 |
url |
http://dx.doi.org/10.1139/m95-107 http://www.nrcresearchpress.com/doi/pdf/10.1139/m95-107 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Canadian Journal of Microbiology volume 41, issue 9, page 776-784 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m95-107 |
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Canadian Journal of Microbiology |
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41 |
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9 |
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776 |
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784 |
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1809895396495851520 |