Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates
The polymerase chain reaction was used to amplify the spacer region located between the 16S and 23S ribosomal RNA genes of strains of Pseudomonas fluorescens and Pseudomonas putida isolated from peat bog, canola field, or arctic plants. Some of these amplified spacer regions were used to probe South...
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1994
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crcansciencepubl:10.1139/m94-087 2023-12-17T10:26:32+01:00 Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates Gill, Santokh Belles-Isles, Julie Brown, Gerry Gagné, Serge Lemieux, Claude Mercier, Jean-Patrick Dion, Patrice 1994 http://dx.doi.org/10.1139/m94-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-087 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 40, issue 7, page 541-547 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1994 crcansciencepubl https://doi.org/10.1139/m94-087 2023-11-19T13:38:37Z The polymerase chain reaction was used to amplify the spacer region located between the 16S and 23S ribosomal RNA genes of strains of Pseudomonas fluorescens and Pseudomonas putida isolated from peat bog, canola field, or arctic plants. Some of these amplified spacer regions were used to probe Southern blots of total DNA digests from the various strains of P. fluorescens and P. putida. Differences were observed in the patterns of hybridization of the various bacterial DNAs. The ribosomal DNA spacer region of four of the P. fluorescens strains examined, strains 64-3, 63-28, QP5, and R17-FP2, was about 515 base pairs (bp) in length, and contained the genes for tRNA Ile and tRNA Ala . The DNA sequences of two strains from canola, 64-3 and 63-28, differed at only two positions. The sequences of the peat bog strains QP5 and R17-FP2 were identical. However, differences were noted between the DNA sequence common to the pair of strains 64-3 and 63-28 and the corresponding common sequence for strains QP5 and R17-FP2. These differences were mainly concentrated in two DNA segments of 10 and 19 bp, respectively. A probe for the 19-bp variable segment that occurs in the ribosomal spacer of strains QP5 and R17-FP2 recognized total DNA from these two strains, but not DNA from other bacteria of different origins. These results suggest the existence of a limited degree of variability within the 16S-23S ribosomal DNA spacer region, and that this variability may be useful to the recognition of particular Pseudomonas strains from environmental samples.Key words: ribosomal RNA genes, intergenic spacer, Pseudomonas soil isolates, specific DNA probe. Article in Journal/Newspaper Arctic Canadian Science Publishing (via Crossref) Arctic Canadian Journal of Microbiology 40 7 541 547 |
institution |
Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Gill, Santokh Belles-Isles, Julie Brown, Gerry Gagné, Serge Lemieux, Claude Mercier, Jean-Patrick Dion, Patrice Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
The polymerase chain reaction was used to amplify the spacer region located between the 16S and 23S ribosomal RNA genes of strains of Pseudomonas fluorescens and Pseudomonas putida isolated from peat bog, canola field, or arctic plants. Some of these amplified spacer regions were used to probe Southern blots of total DNA digests from the various strains of P. fluorescens and P. putida. Differences were observed in the patterns of hybridization of the various bacterial DNAs. The ribosomal DNA spacer region of four of the P. fluorescens strains examined, strains 64-3, 63-28, QP5, and R17-FP2, was about 515 base pairs (bp) in length, and contained the genes for tRNA Ile and tRNA Ala . The DNA sequences of two strains from canola, 64-3 and 63-28, differed at only two positions. The sequences of the peat bog strains QP5 and R17-FP2 were identical. However, differences were noted between the DNA sequence common to the pair of strains 64-3 and 63-28 and the corresponding common sequence for strains QP5 and R17-FP2. These differences were mainly concentrated in two DNA segments of 10 and 19 bp, respectively. A probe for the 19-bp variable segment that occurs in the ribosomal spacer of strains QP5 and R17-FP2 recognized total DNA from these two strains, but not DNA from other bacteria of different origins. These results suggest the existence of a limited degree of variability within the 16S-23S ribosomal DNA spacer region, and that this variability may be useful to the recognition of particular Pseudomonas strains from environmental samples.Key words: ribosomal RNA genes, intergenic spacer, Pseudomonas soil isolates, specific DNA probe. |
format |
Article in Journal/Newspaper |
author |
Gill, Santokh Belles-Isles, Julie Brown, Gerry Gagné, Serge Lemieux, Claude Mercier, Jean-Patrick Dion, Patrice |
author_facet |
Gill, Santokh Belles-Isles, Julie Brown, Gerry Gagné, Serge Lemieux, Claude Mercier, Jean-Patrick Dion, Patrice |
author_sort |
Gill, Santokh |
title |
Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
title_short |
Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
title_full |
Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
title_fullStr |
Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
title_full_unstemmed |
Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates |
title_sort |
identification of variability of ribosomal dna spacer from pseudomonas soil isolates |
publisher |
Canadian Science Publishing |
publishDate |
1994 |
url |
http://dx.doi.org/10.1139/m94-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-087 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Canadian Journal of Microbiology volume 40, issue 7, page 541-547 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m94-087 |
container_title |
Canadian Journal of Microbiology |
container_volume |
40 |
container_issue |
7 |
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541 |
op_container_end_page |
547 |
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1785578260498743296 |