Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean
Eighty regional strains of Vibrio isolated from the seasonally cold waters of coastal Newfoundland, and a number of Vibrio reference cultures, were studied. The regional strains had been isolated from the brown macroalga Alaria esculenta and the giant scallop Placopecten magellanicus and were known...
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1994
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Online Access: | http://dx.doi.org/10.1139/m94-073 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-073 |
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crcansciencepubl:10.1139/m94-073 2024-03-03T08:46:44+00:00 Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean Martin-Kearley, Jennifer Gow, John A. Péloquin, Marc Greer, Charles W. 1994 http://dx.doi.org/10.1139/m94-073 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-073 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 40, issue 6, page 446-455 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1994 crcansciencepubl https://doi.org/10.1139/m94-073 2024-02-07T10:53:43Z Eighty regional strains of Vibrio isolated from the seasonally cold waters of coastal Newfoundland, and a number of Vibrio reference cultures, were studied. The regional strains had been isolated from the brown macroalga Alaria esculenta and the giant scallop Placopecten magellanicus and were known to grow at 4 °C. The strains were grouped according to their arginine-dihydrolase reactions and examined by numerical analysis. According to phenotypic properties the arginine-dihydrolase positive strains closely resembled Vibrio splendidus biovar I. Most clusters of the arginine-dihydrolase negative strains appeared to be unique but the closest phenotypic resemblance among some strains was with Vibrio ordalii. Some strains were examined using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique for fingerprinting and it was shown that the regional strains were significantly different from either V. splendidus biovar I or V. ordalii. Generally, the strains from seaweed clustered separately from those that were from scallops. Strains in some clusters, especially those from the seaweed, were able to utilize most of the compounds that were tested as sole sources of carbon and energy.Key words: numerical taxonomy, marine bacteria, Vibrio, psychrotrophs, RAPD-PCR. Article in Journal/Newspaper Newfoundland Canadian Science Publishing Canadian Journal of Microbiology 40 6 446 455 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
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English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Martin-Kearley, Jennifer Gow, John A. Péloquin, Marc Greer, Charles W. Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Eighty regional strains of Vibrio isolated from the seasonally cold waters of coastal Newfoundland, and a number of Vibrio reference cultures, were studied. The regional strains had been isolated from the brown macroalga Alaria esculenta and the giant scallop Placopecten magellanicus and were known to grow at 4 °C. The strains were grouped according to their arginine-dihydrolase reactions and examined by numerical analysis. According to phenotypic properties the arginine-dihydrolase positive strains closely resembled Vibrio splendidus biovar I. Most clusters of the arginine-dihydrolase negative strains appeared to be unique but the closest phenotypic resemblance among some strains was with Vibrio ordalii. Some strains were examined using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique for fingerprinting and it was shown that the regional strains were significantly different from either V. splendidus biovar I or V. ordalii. Generally, the strains from seaweed clustered separately from those that were from scallops. Strains in some clusters, especially those from the seaweed, were able to utilize most of the compounds that were tested as sole sources of carbon and energy.Key words: numerical taxonomy, marine bacteria, Vibrio, psychrotrophs, RAPD-PCR. |
format |
Article in Journal/Newspaper |
author |
Martin-Kearley, Jennifer Gow, John A. Péloquin, Marc Greer, Charles W. |
author_facet |
Martin-Kearley, Jennifer Gow, John A. Péloquin, Marc Greer, Charles W. |
author_sort |
Martin-Kearley, Jennifer |
title |
Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
title_short |
Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
title_full |
Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
title_fullStr |
Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
title_full_unstemmed |
Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean |
title_sort |
numerical analysis and the application of random amplified polymorphic dna polymerase chain reaction to the differentiation of vibrio strains from a seasonally cold ocean |
publisher |
Canadian Science Publishing |
publishDate |
1994 |
url |
http://dx.doi.org/10.1139/m94-073 http://www.nrcresearchpress.com/doi/pdf/10.1139/m94-073 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Canadian Journal of Microbiology volume 40, issue 6, page 446-455 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m94-073 |
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Canadian Journal of Microbiology |
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40 |
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6 |
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446 |
op_container_end_page |
455 |
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1792502790300368896 |