1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8
The metabolic pathway used by Rhodococcus sp. BPG-8 isolated from oil-rich soil in Newfoundland for the degradation of 1, 3, 5-trihydroxybenzene (phloroglucinol) as a sole source of carbon and energy was determined. Culture filtrate of cells grown on phloroglucinol detected 1, 2, 3, 5-tetrahydroxybe...
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Online Access: | http://dx.doi.org/10.1139/m93-024 http://www.nrcresearchpress.com/doi/pdf/10.1139/m93-024 |
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crcansciencepubl:10.1139/m93-024 2024-03-03T08:46:44+00:00 1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 Armstrong, S. Patel, T. R. 1993 http://dx.doi.org/10.1139/m93-024 http://www.nrcresearchpress.com/doi/pdf/10.1139/m93-024 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 39, issue 2, page 175-179 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1993 crcansciencepubl https://doi.org/10.1139/m93-024 2024-02-07T10:53:37Z The metabolic pathway used by Rhodococcus sp. BPG-8 isolated from oil-rich soil in Newfoundland for the degradation of 1, 3, 5-trihydroxybenzene (phloroglucinol) as a sole source of carbon and energy was determined. Culture filtrate of cells grown on phloroglucinol detected 1, 2, 3, 5-tetrahydroxybenzene when extracted and analyzed using gas chromatography - mass spectrometry, thin-layer chromatography, and ultraviolet spectrophotometry. Nonaromatics were either derivatized with 2, 4-dinitrophenylhydrazine and compared against authentic standards by the above methods, or detected by chemical and enzymatic methods. Extracts of cells grown on phloroglucinol contained phloroglucinol hydroxylase activity, and a dioxygenase that carried out ortho-cleavage of 1, 2, 3, 5-tetrahydroxybenzene. The extract also showed inducible activity for further metabolism of acetopyruvate leading to accumulation of formate in the supernatant. A tentative degradative pathway for phloroglucinol by Rhodococcus sp. BPG—8 is proposed.Key words: phloroglucinol, 1, 2, 3, 5-tetrahydroxybenzene, degradation, Rhodococcus. Article in Journal/Newspaper Newfoundland Canadian Science Publishing Canadian Journal of Microbiology 39 2 175 179 |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Armstrong, S. Patel, T. R. 1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
The metabolic pathway used by Rhodococcus sp. BPG-8 isolated from oil-rich soil in Newfoundland for the degradation of 1, 3, 5-trihydroxybenzene (phloroglucinol) as a sole source of carbon and energy was determined. Culture filtrate of cells grown on phloroglucinol detected 1, 2, 3, 5-tetrahydroxybenzene when extracted and analyzed using gas chromatography - mass spectrometry, thin-layer chromatography, and ultraviolet spectrophotometry. Nonaromatics were either derivatized with 2, 4-dinitrophenylhydrazine and compared against authentic standards by the above methods, or detected by chemical and enzymatic methods. Extracts of cells grown on phloroglucinol contained phloroglucinol hydroxylase activity, and a dioxygenase that carried out ortho-cleavage of 1, 2, 3, 5-tetrahydroxybenzene. The extract also showed inducible activity for further metabolism of acetopyruvate leading to accumulation of formate in the supernatant. A tentative degradative pathway for phloroglucinol by Rhodococcus sp. BPG—8 is proposed.Key words: phloroglucinol, 1, 2, 3, 5-tetrahydroxybenzene, degradation, Rhodococcus. |
format |
Article in Journal/Newspaper |
author |
Armstrong, S. Patel, T. R. |
author_facet |
Armstrong, S. Patel, T. R. |
author_sort |
Armstrong, S. |
title |
1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
title_short |
1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
title_full |
1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
title_fullStr |
1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
title_full_unstemmed |
1, 3, 5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8 |
title_sort |
1, 3, 5-trihydroxybenzene biodegradation by rhodococcus sp. bpg-8 |
publisher |
Canadian Science Publishing |
publishDate |
1993 |
url |
http://dx.doi.org/10.1139/m93-024 http://www.nrcresearchpress.com/doi/pdf/10.1139/m93-024 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Canadian Journal of Microbiology volume 39, issue 2, page 175-179 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m93-024 |
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Canadian Journal of Microbiology |
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39 |
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2 |
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175 |
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179 |
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1792502780977479680 |