Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton

• Published in: Canadian Journal of Microbiology
• Main Authors: Hollibaugh, James T., Wong, Patricia S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 1992
• Subjects: Palmer Station; Palmer-Station; Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1139/m92-100; http://www.nrcresearchpress.com/doi/pdf/10.1139/m92-100

Bacterioplankton productivity measurements based on [methyl- 3 H]-thymidine (TdR) or L-[3,4,5- 3 H]leucine (L-leu) incorporation typically depend on cold trichloroacetic acid (TCA) precipitation to separate 3 H uptake from incorporation. An additional rinse with cold 80% ethanol (EtOH) removed an av...