Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton
Bacterioplankton productivity measurements based on [methyl- 3 H]-thymidine (TdR) or L-[3,4,5- 3 H]leucine (L-leu) incorporation typically depend on cold trichloroacetic acid (TCA) precipitation to separate 3 H uptake from incorporation. An additional rinse with cold 80% ethanol (EtOH) removed an av...
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Online Access: | http://dx.doi.org/10.1139/m92-100 http://www.nrcresearchpress.com/doi/pdf/10.1139/m92-100 |
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crcansciencepubl:10.1139/m92-100 2024-09-30T14:26:01+00:00 Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton Hollibaugh, James T. Wong, Patricia S. 1992 http://dx.doi.org/10.1139/m92-100 http://www.nrcresearchpress.com/doi/pdf/10.1139/m92-100 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 38, issue 7, page 605-613 ISSN 0008-4166 1480-3275 journal-article 1992 crcansciencepubl https://doi.org/10.1139/m92-100 2024-09-19T04:09:49Z Bacterioplankton productivity measurements based on [methyl- 3 H]-thymidine (TdR) or L-[3,4,5- 3 H]leucine (L-leu) incorporation typically depend on cold trichloroacetic acid (TCA) precipitation to separate 3 H uptake from incorporation. An additional rinse with cold 80% ethanol (EtOH) removed an average of 22 (L-leu) and 32% (TdR) of 3 H "incorporated" by San Francisco Bay samples and decreased the between-duplicate difference by a factor of 3.5. Similar results were obtained with samples from Tomales Bay, Calif., and Palmer Station, Antarctica. Varying amounts of cold TCA insoluble radiolabel from six other substrates were removed by the EtOH rinse. Regression analysis showed relationships between the effect of the EtOH rinse and a group of environmental variables and derived parameters. The percentage of 3 H removed was generally independent of filter type; however, there were often large differences in the amount of 3 H retained by Millipore versus Nuclepore or Poretics filters. The results strongly suggest that an EtOH rinse or other organic extraction should be included in protocols to determine incorporation of radiolabeled substrates into macromolecules. Furthermore, sequestering low molecular weight substrates in some sort of lipid-bound pool may represent a general storage mechanism employed by bacterioplankton. Key words: bacterioplankton, production, San Francisco Bay, filtration, incorporation. Article in Journal/Newspaper Antarc* Antarctica Canadian Science Publishing Palmer Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer-Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Canadian Journal of Microbiology 38 7 605 613 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
Bacterioplankton productivity measurements based on [methyl- 3 H]-thymidine (TdR) or L-[3,4,5- 3 H]leucine (L-leu) incorporation typically depend on cold trichloroacetic acid (TCA) precipitation to separate 3 H uptake from incorporation. An additional rinse with cold 80% ethanol (EtOH) removed an average of 22 (L-leu) and 32% (TdR) of 3 H "incorporated" by San Francisco Bay samples and decreased the between-duplicate difference by a factor of 3.5. Similar results were obtained with samples from Tomales Bay, Calif., and Palmer Station, Antarctica. Varying amounts of cold TCA insoluble radiolabel from six other substrates were removed by the EtOH rinse. Regression analysis showed relationships between the effect of the EtOH rinse and a group of environmental variables and derived parameters. The percentage of 3 H removed was generally independent of filter type; however, there were often large differences in the amount of 3 H retained by Millipore versus Nuclepore or Poretics filters. The results strongly suggest that an EtOH rinse or other organic extraction should be included in protocols to determine incorporation of radiolabeled substrates into macromolecules. Furthermore, sequestering low molecular weight substrates in some sort of lipid-bound pool may represent a general storage mechanism employed by bacterioplankton. Key words: bacterioplankton, production, San Francisco Bay, filtration, incorporation. |
format |
Article in Journal/Newspaper |
author |
Hollibaugh, James T. Wong, Patricia S. |
spellingShingle |
Hollibaugh, James T. Wong, Patricia S. Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
author_facet |
Hollibaugh, James T. Wong, Patricia S. |
author_sort |
Hollibaugh, James T. |
title |
Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
title_short |
Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
title_full |
Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
title_fullStr |
Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
title_full_unstemmed |
Ethanol-extractable substrate pools and the incorporation of thymidine, L-leucine, and other substrates by bacterioplankton |
title_sort |
ethanol-extractable substrate pools and the incorporation of thymidine, l-leucine, and other substrates by bacterioplankton |
publisher |
Canadian Science Publishing |
publishDate |
1992 |
url |
http://dx.doi.org/10.1139/m92-100 http://www.nrcresearchpress.com/doi/pdf/10.1139/m92-100 |
long_lat |
ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-64.050,-64.050,-64.770,-64.770) |
geographic |
Palmer Station Palmer-Station |
geographic_facet |
Palmer Station Palmer-Station |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Canadian Journal of Microbiology volume 38, issue 7, page 605-613 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m92-100 |
container_title |
Canadian Journal of Microbiology |
container_volume |
38 |
container_issue |
7 |
container_start_page |
605 |
op_container_end_page |
613 |
_version_ |
1811646546270224384 |