The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus
Murine neuroblastoma (NA-C1300) and baby hamster kidney (BHK-21/C13) cell cultures were infected with the Canadian Arctic strain of rabies virus. Subcultures were passed following incubation for 3 to 4 days at 35 °C. The supernatant fluids from the BHK cultures demonstrated increasing infectivity in...
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Canadian Science Publishing
1989
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Online Access: | http://dx.doi.org/10.1139/m89-135 http://www.nrcresearchpress.com/doi/pdf/10.1139/m89-135 |
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crcansciencepubl:10.1139/m89-135 2023-12-17T10:25:26+01:00 The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus Webster, W. A. Charlton, K. M. 1989 http://dx.doi.org/10.1139/m89-135 http://www.nrcresearchpress.com/doi/pdf/10.1139/m89-135 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 35, issue 8, page 811-813 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1989 crcansciencepubl https://doi.org/10.1139/m89-135 2023-11-19T13:39:19Z Murine neuroblastoma (NA-C1300) and baby hamster kidney (BHK-21/C13) cell cultures were infected with the Canadian Arctic strain of rabies virus. Subcultures were passed following incubation for 3 to 4 days at 35 °C. The supernatant fluids from the BHK cultures demonstrated increasing infectivity in both NA and BHK cells concomitantly with an increase in the number of parent cells staining with an anti-glycoprotein stain. On the other hand, the supernatant fluids from the NA cultures initially showed higher infectivity in NA cells than in BHK cells. This feature was related to a low production of glycoprotein-staining cells in the parent NA cultures. The reduction of infectivity in NA cells of some NA supernatant fluids (and brain suspensions) by anti-nucleoprotein antibodies suggests that nucleocapsid material is, in some manner, capable of infecting NA cells. Infectivity of this virus strain in experimental mice is also related to the production of glycoprotein and may not be correlated with the degree of infection in NA cell cultures.Key words: rabies, nucleocapsid, infection, cells. Article in Journal/Newspaper Arctic Canadian Science Publishing (via Crossref) Arctic Canadian Journal of Microbiology 35 8 811 813 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Webster, W. A. Charlton, K. M. The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Murine neuroblastoma (NA-C1300) and baby hamster kidney (BHK-21/C13) cell cultures were infected with the Canadian Arctic strain of rabies virus. Subcultures were passed following incubation for 3 to 4 days at 35 °C. The supernatant fluids from the BHK cultures demonstrated increasing infectivity in both NA and BHK cells concomitantly with an increase in the number of parent cells staining with an anti-glycoprotein stain. On the other hand, the supernatant fluids from the NA cultures initially showed higher infectivity in NA cells than in BHK cells. This feature was related to a low production of glycoprotein-staining cells in the parent NA cultures. The reduction of infectivity in NA cells of some NA supernatant fluids (and brain suspensions) by anti-nucleoprotein antibodies suggests that nucleocapsid material is, in some manner, capable of infecting NA cells. Infectivity of this virus strain in experimental mice is also related to the production of glycoprotein and may not be correlated with the degree of infection in NA cell cultures.Key words: rabies, nucleocapsid, infection, cells. |
format |
Article in Journal/Newspaper |
author |
Webster, W. A. Charlton, K. M. |
author_facet |
Webster, W. A. Charlton, K. M. |
author_sort |
Webster, W. A. |
title |
The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
title_short |
The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
title_full |
The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
title_fullStr |
The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
title_full_unstemmed |
The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus |
title_sort |
apparent infection of na-c1300 cell cultures by nucleocapsid material of the canadian arctic strain of rabies virus |
publisher |
Canadian Science Publishing |
publishDate |
1989 |
url |
http://dx.doi.org/10.1139/m89-135 http://www.nrcresearchpress.com/doi/pdf/10.1139/m89-135 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Canadian Journal of Microbiology volume 35, issue 8, page 811-813 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m89-135 |
container_title |
Canadian Journal of Microbiology |
container_volume |
35 |
container_issue |
8 |
container_start_page |
811 |
op_container_end_page |
813 |
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1785576748831735808 |