Tick-borne Kemerovo group orbiviruses in a Newfoundland seabird colony

• Published in: Canadian Journal of Microbiology
• Main Authors: Oprandy, John J., Schwan, Tom G., Main, Andrew J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 1988
• Subjects: Genetics; Molecular Biology; Applied Microbiology and Biotechnology; General Medicine; Immunology; Microbiology; Canada; Newfoundland
• Online Access: http://dx.doi.org/10.1139/m88-133; http://www.nrcresearchpress.com/doi/pdf/10.1139/m88-133

Five new isolates of Kemerovo group viruses were recovered from Ixodes uriae collected on Great Island, Witless Bay Seabird Sanctuary, Newfoundland, Canada, during July 1985. This brings the total number of Orbivirus isolates on Great Island to 18 isolates including the 7 from 1971 and 6 from 1972. Genomic segments of several strains were compared by polyacrylamide gel electrophoresis. The degree of variation in each segment of these viruses was calculated. Great Island and Bauline viruses exhibited a great degree of variation in dsRNA migration patterns. Great Island and Bauline genomes averaged 11.60 (SD = 0.107) and 11.69 megadaltons (SD = 0.075), respectively. Variation was observed in all 10 segments of Great Island and Bauline viruses. These findings were compared with serologic and protein gel data.