Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria
Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degr...
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Canadian Science Publishing
1983
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Online Access: | http://dx.doi.org/10.1139/m83-079 http://www.nrcresearchpress.com/doi/pdf/10.1139/m83-079 |
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crcansciencepubl:10.1139/m83-079 2024-04-07T07:55:34+00:00 Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria Fedorak, P. M. Westlake, D. W. S. 1983 http://dx.doi.org/10.1139/m83-079 http://www.nrcresearchpress.com/doi/pdf/10.1139/m83-079 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 29, issue 5, page 497-503 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1983 crcansciencepubl https://doi.org/10.1139/m83-079 2024-03-08T00:37:43Z Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degraded only biphenyl, 3-methylbiphenyl, 4-methylbiphenyl, and three other minor, unidentified compounds in the aromatic fraction. No degradation was detected in the saturate fraction, nor in the sulfur heterocyclic component of the oil. When grown on any of the pure biphenyls, these isolates produced a transient, yellow intermediate which had the spectrophotometric characteristics of α-hydroxymuconic semialdehydes. Growth on either methylbiphenyl compound produced a methylbenzoic acid, indicating that the nonmethylated ring was the first to be cleaved. The isolates, identified as Alcaligenes sp. and Acinetobacter sp., were also able to grow on benzoic acid, 3-, and 4-methylbenzoic acids, indicating that they could further metabolize the aromatic acid intermediates. Article in Journal/Newspaper Prudhoe Bay Canadian Science Publishing Canadian Journal of Microbiology 29 5 497 503 |
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Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Fedorak, P. M. Westlake, D. W. S. Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degraded only biphenyl, 3-methylbiphenyl, 4-methylbiphenyl, and three other minor, unidentified compounds in the aromatic fraction. No degradation was detected in the saturate fraction, nor in the sulfur heterocyclic component of the oil. When grown on any of the pure biphenyls, these isolates produced a transient, yellow intermediate which had the spectrophotometric characteristics of α-hydroxymuconic semialdehydes. Growth on either methylbiphenyl compound produced a methylbenzoic acid, indicating that the nonmethylated ring was the first to be cleaved. The isolates, identified as Alcaligenes sp. and Acinetobacter sp., were also able to grow on benzoic acid, 3-, and 4-methylbenzoic acids, indicating that they could further metabolize the aromatic acid intermediates. |
format |
Article in Journal/Newspaper |
author |
Fedorak, P. M. Westlake, D. W. S. |
author_facet |
Fedorak, P. M. Westlake, D. W. S. |
author_sort |
Fedorak, P. M. |
title |
Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
title_short |
Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
title_full |
Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
title_fullStr |
Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
title_full_unstemmed |
Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
title_sort |
selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria |
publisher |
Canadian Science Publishing |
publishDate |
1983 |
url |
http://dx.doi.org/10.1139/m83-079 http://www.nrcresearchpress.com/doi/pdf/10.1139/m83-079 |
genre |
Prudhoe Bay |
genre_facet |
Prudhoe Bay |
op_source |
Canadian Journal of Microbiology volume 29, issue 5, page 497-503 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m83-079 |
container_title |
Canadian Journal of Microbiology |
container_volume |
29 |
container_issue |
5 |
container_start_page |
497 |
op_container_end_page |
503 |
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1795672809084551168 |