Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil
Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, s...
Published in: | Canadian Journal of Microbiology |
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1978
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Online Access: | http://dx.doi.org/10.1139/m78-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/m78-236 |
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crcansciencepubl:10.1139/m78-236 2023-12-17T10:26:13+01:00 Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil Nelson, Louise M. Parkinson, D. 1978 http://dx.doi.org/10.1139/m78-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/m78-236 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 24, issue 12, page 1468-1474 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1978 crcansciencepubl https://doi.org/10.1139/m78-236 2023-11-19T13:38:37Z Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. Pseudomonas M216 was most susceptible to freeze–thaw damage under these conditions. Arthrobacter M51 was the most resistant of the three isolates when frozen in sand or soil and when frozen at a high rate after growth at varing rates at 5 and 15 °C in carbon- or nitrogen-limited media. Pseudomonas M216 was more sensitive to freeze-thaw damage when NaCl was present in the freezing menstruum, even at low freezing rates. Survival of cells frozen in growth medium, water, saline, and soil extract was not affected by the freezing rate when it was less than 1 °C min −1 . Soil extract did not protect cells from freeze–thaw damage any more than water and for Arthrobacter M51 survival was decreased when it was frozen in soil extract. Article in Journal/Newspaper Arctic Devon Island Canadian Science Publishing (via Crossref) Arctic Canada Devon Island ENVELOPE(-88.000,-88.000,75.252,75.252) Canadian Journal of Microbiology 24 12 1468 1474 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Nelson, Louise M. Parkinson, D. Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. Pseudomonas M216 was most susceptible to freeze–thaw damage under these conditions. Arthrobacter M51 was the most resistant of the three isolates when frozen in sand or soil and when frozen at a high rate after growth at varing rates at 5 and 15 °C in carbon- or nitrogen-limited media. Pseudomonas M216 was more sensitive to freeze-thaw damage when NaCl was present in the freezing menstruum, even at low freezing rates. Survival of cells frozen in growth medium, water, saline, and soil extract was not affected by the freezing rate when it was less than 1 °C min −1 . Soil extract did not protect cells from freeze–thaw damage any more than water and for Arthrobacter M51 survival was decreased when it was frozen in soil extract. |
format |
Article in Journal/Newspaper |
author |
Nelson, Louise M. Parkinson, D. |
author_facet |
Nelson, Louise M. Parkinson, D. |
author_sort |
Nelson, Louise M. |
title |
Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
title_short |
Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
title_full |
Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
title_fullStr |
Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
title_full_unstemmed |
Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
title_sort |
effect of freezing and thawing on survival of three bacterial isolates from an arctic soil |
publisher |
Canadian Science Publishing |
publishDate |
1978 |
url |
http://dx.doi.org/10.1139/m78-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/m78-236 |
long_lat |
ENVELOPE(-88.000,-88.000,75.252,75.252) |
geographic |
Arctic Canada Devon Island |
geographic_facet |
Arctic Canada Devon Island |
genre |
Arctic Devon Island |
genre_facet |
Arctic Devon Island |
op_source |
Canadian Journal of Microbiology volume 24, issue 12, page 1468-1474 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m78-236 |
container_title |
Canadian Journal of Microbiology |
container_volume |
24 |
container_issue |
12 |
container_start_page |
1468 |
op_container_end_page |
1474 |
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1785577926508412928 |