Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture
Three bacterial isolates from an arctic meadow soil, Pseudomonas M216, Bacillus M153, and Arthrobacter M51, were grown continuously in carbon-and nitrogen-limiting media at 15 and 5 °C at three dilution rates from 0.04 to 0.01 h −1 . Measurements of yield, viability, endogenous oxygen-uptake rate, a...
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crcansciencepubl:10.1139/m78-234 2023-12-17T10:25:36+01:00 Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture Nelson, Louise M. 1978 http://dx.doi.org/10.1139/m78-234 http://www.nrcresearchpress.com/doi/pdf/10.1139/m78-234 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 24, issue 12, page 1452-1459 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1978 crcansciencepubl https://doi.org/10.1139/m78-234 2023-11-19T13:38:49Z Three bacterial isolates from an arctic meadow soil, Pseudomonas M216, Bacillus M153, and Arthrobacter M51, were grown continuously in carbon-and nitrogen-limiting media at 15 and 5 °C at three dilution rates from 0.04 to 0.01 h −1 . Measurements of yield, viability, endogenous oxygen-uptake rate, and cell composition indicated that in general, these isolates were well adapted for growth at low rates and low temperature under nutrient limitation. Changes in cell composition with temperature, growth rate, and nutrient limitation followed patterns similar to those observed in organisms studied in other laboratories. Yields were higher at 15 than at 5 °C in Pseudomonas M216 and Arthrobacter M51 and the endogenous respiration rate tended to decrease with decreasing dilution rate. Substrate affinity (K s ) and μ max varied with temperature, and Pseudomonas M216 exhibited the lowest K s for carbon and nitrogen and highest μ max under the growth conditions studied, except at 15 °C under carbon limitation where Arthrobacter M51 exhibited the lowest K s . Only Bacillus M153 exhibited a significant loss in viability at low dilution rates and a detectable specific maintenance rate (0.0077 h −1 ), factors which may contribute to the low isolation frequency of the genus at the meadow site. Article in Journal/Newspaper Arctic Canadian Science Publishing (via Crossref) Arctic Canadian Journal of Microbiology 24 12 1452 1459 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Nelson, Louise M. Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Three bacterial isolates from an arctic meadow soil, Pseudomonas M216, Bacillus M153, and Arthrobacter M51, were grown continuously in carbon-and nitrogen-limiting media at 15 and 5 °C at three dilution rates from 0.04 to 0.01 h −1 . Measurements of yield, viability, endogenous oxygen-uptake rate, and cell composition indicated that in general, these isolates were well adapted for growth at low rates and low temperature under nutrient limitation. Changes in cell composition with temperature, growth rate, and nutrient limitation followed patterns similar to those observed in organisms studied in other laboratories. Yields were higher at 15 than at 5 °C in Pseudomonas M216 and Arthrobacter M51 and the endogenous respiration rate tended to decrease with decreasing dilution rate. Substrate affinity (K s ) and μ max varied with temperature, and Pseudomonas M216 exhibited the lowest K s for carbon and nitrogen and highest μ max under the growth conditions studied, except at 15 °C under carbon limitation where Arthrobacter M51 exhibited the lowest K s . Only Bacillus M153 exhibited a significant loss in viability at low dilution rates and a detectable specific maintenance rate (0.0077 h −1 ), factors which may contribute to the low isolation frequency of the genus at the meadow site. |
format |
Article in Journal/Newspaper |
author |
Nelson, Louise M. |
author_facet |
Nelson, Louise M. |
author_sort |
Nelson, Louise M. |
title |
Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
title_short |
Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
title_full |
Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
title_fullStr |
Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
title_full_unstemmed |
Effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
title_sort |
effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture |
publisher |
Canadian Science Publishing |
publishDate |
1978 |
url |
http://dx.doi.org/10.1139/m78-234 http://www.nrcresearchpress.com/doi/pdf/10.1139/m78-234 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Canadian Journal of Microbiology volume 24, issue 12, page 1452-1459 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m78-234 |
container_title |
Canadian Journal of Microbiology |
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24 |
container_issue |
12 |
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1452 |
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1459 |
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1785577226538844160 |