The fluorescent antibody test for the identification of strains of Clostridium botulinum type E
A fluorescent antibody test for the identification of toxigenic and nontoxigenic type E strains of C. botulinum is described. Hyperimmune rabbit sera were prepared to cell suspensions of the D8, 070, and Beluga strains and to spores of the D8 and PM-15 strains. Fluorescein isothiocyanate immunoglobu...
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1970
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crcansciencepubl:10.1139/m70-158 2023-12-17T10:28:03+01:00 The fluorescent antibody test for the identification of strains of Clostridium botulinum type E Emeruwa, A. C. Ashton, F. E. Hawirko, R. Z. 1970 http://dx.doi.org/10.1139/m70-158 http://www.nrcresearchpress.com/doi/pdf/10.1139/m70-158 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 16, issue 10, page 917-921 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1970 crcansciencepubl https://doi.org/10.1139/m70-158 2023-11-19T13:39:30Z A fluorescent antibody test for the identification of toxigenic and nontoxigenic type E strains of C. botulinum is described. Hyperimmune rabbit sera were prepared to cell suspensions of the D8, 070, and Beluga strains and to spores of the D8 and PM-15 strains. Fluorescein isothiocyanate immunoglobulin conjugates were prepared and absorbed with C. bifermentans and diluted to eliminate non-specific staining and cross-reactivity with related serotypes. Enzyme pretreatment of spores and washing the stained spores in carbonate buffer, pH 9.0, enhanced the sensitivity of the test. Conjugates of cell antiserum, in most cases, stained the spore bodies and not the vegetative cells of the cross-reacting strains. Conjugates of spore antiserum stained vegetative cells as well as spores but the fluorescence of the cells was eliminated by absorption of the conjugates with young cells of the homologous strain. The absorbed conjugates stained spores of 19/20 isolates from diverse geographical areas without any reduction in the intensity of fluorescence, indicating that spore antigens carry the determinant groups of type E specificity and that the common antigen is a component of the spore body. In addition, some of the cross-reacting strains showed the same degree of fluorescence as the homologous strain, suggesting that more than one antigen may be shared by these strains. Article in Journal/Newspaper Beluga Beluga* Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 16 10 917 921 |
institution |
Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Emeruwa, A. C. Ashton, F. E. Hawirko, R. Z. The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
A fluorescent antibody test for the identification of toxigenic and nontoxigenic type E strains of C. botulinum is described. Hyperimmune rabbit sera were prepared to cell suspensions of the D8, 070, and Beluga strains and to spores of the D8 and PM-15 strains. Fluorescein isothiocyanate immunoglobulin conjugates were prepared and absorbed with C. bifermentans and diluted to eliminate non-specific staining and cross-reactivity with related serotypes. Enzyme pretreatment of spores and washing the stained spores in carbonate buffer, pH 9.0, enhanced the sensitivity of the test. Conjugates of cell antiserum, in most cases, stained the spore bodies and not the vegetative cells of the cross-reacting strains. Conjugates of spore antiserum stained vegetative cells as well as spores but the fluorescence of the cells was eliminated by absorption of the conjugates with young cells of the homologous strain. The absorbed conjugates stained spores of 19/20 isolates from diverse geographical areas without any reduction in the intensity of fluorescence, indicating that spore antigens carry the determinant groups of type E specificity and that the common antigen is a component of the spore body. In addition, some of the cross-reacting strains showed the same degree of fluorescence as the homologous strain, suggesting that more than one antigen may be shared by these strains. |
format |
Article in Journal/Newspaper |
author |
Emeruwa, A. C. Ashton, F. E. Hawirko, R. Z. |
author_facet |
Emeruwa, A. C. Ashton, F. E. Hawirko, R. Z. |
author_sort |
Emeruwa, A. C. |
title |
The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
title_short |
The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
title_full |
The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
title_fullStr |
The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
title_full_unstemmed |
The fluorescent antibody test for the identification of strains of Clostridium botulinum type E |
title_sort |
fluorescent antibody test for the identification of strains of clostridium botulinum type e |
publisher |
Canadian Science Publishing |
publishDate |
1970 |
url |
http://dx.doi.org/10.1139/m70-158 http://www.nrcresearchpress.com/doi/pdf/10.1139/m70-158 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Canadian Journal of Microbiology volume 16, issue 10, page 917-921 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m70-158 |
container_title |
Canadian Journal of Microbiology |
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16 |
container_issue |
10 |
container_start_page |
917 |
op_container_end_page |
921 |
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1785580065767030784 |