Sorption of water by spores, heat-killed spores, and vegetative cells
Several hypotheses explain the heat resistance of bacterial spores in terms of a hydrophobic nature of the spore surface and possibly also the spore interior. The water-sorption properties of naturally hydrated spores which had never been dehydrated before the experiment were studied. The rate of lo...
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crcansciencepubl:10.1139/m70-096 2023-12-17T10:28:02+01:00 Sorption of water by spores, heat-killed spores, and vegetative cells Grecz, Nicholas Smith, Roberta F. Hoffmann, Charles C. 1970 http://dx.doi.org/10.1139/m70-096 http://www.nrcresearchpress.com/doi/pdf/10.1139/m70-096 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 16, issue 7, page 573-579 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1970 crcansciencepubl https://doi.org/10.1139/m70-096 2023-11-19T13:38:13Z Several hypotheses explain the heat resistance of bacterial spores in terms of a hydrophobic nature of the spore surface and possibly also the spore interior. The water-sorption properties of naturally hydrated spores which had never been dehydrated before the experiment were studied. The rate of loss of water over P 2 O 5 at 50 °C was measured in a closed chamber by remote weighing with a Cahn electrobalance. The hygroscopicity expressed as percentage of water bound by the sample at a w = 1, 25 °C was as follows.(I) Chemicals:albumin, 70.5; starch, 42.9.(II) Clostridium botulinum 33A, a heat-resistant strain: spores, 47.0; residue (spores heat killed at 121 °C for 30 min), 50.4; exudate (material released from heat-killed spores) 63.1; vegetative cells, 70.3.(III) C. botulinum, type E, strain Beluga, a heat-sensitive strain: spores 62.5; residue, 61.3; exudate, 77.3.It is postulated that molecular masking in the spore is responsible for low binding of water, electrical and chemical inertness, biological dormancy, and high heat resistance of bacterial spores. Article in Journal/Newspaper Beluga Beluga* Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 16 7 573 579 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Grecz, Nicholas Smith, Roberta F. Hoffmann, Charles C. Sorption of water by spores, heat-killed spores, and vegetative cells |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Several hypotheses explain the heat resistance of bacterial spores in terms of a hydrophobic nature of the spore surface and possibly also the spore interior. The water-sorption properties of naturally hydrated spores which had never been dehydrated before the experiment were studied. The rate of loss of water over P 2 O 5 at 50 °C was measured in a closed chamber by remote weighing with a Cahn electrobalance. The hygroscopicity expressed as percentage of water bound by the sample at a w = 1, 25 °C was as follows.(I) Chemicals:albumin, 70.5; starch, 42.9.(II) Clostridium botulinum 33A, a heat-resistant strain: spores, 47.0; residue (spores heat killed at 121 °C for 30 min), 50.4; exudate (material released from heat-killed spores) 63.1; vegetative cells, 70.3.(III) C. botulinum, type E, strain Beluga, a heat-sensitive strain: spores 62.5; residue, 61.3; exudate, 77.3.It is postulated that molecular masking in the spore is responsible for low binding of water, electrical and chemical inertness, biological dormancy, and high heat resistance of bacterial spores. |
format |
Article in Journal/Newspaper |
author |
Grecz, Nicholas Smith, Roberta F. Hoffmann, Charles C. |
author_facet |
Grecz, Nicholas Smith, Roberta F. Hoffmann, Charles C. |
author_sort |
Grecz, Nicholas |
title |
Sorption of water by spores, heat-killed spores, and vegetative cells |
title_short |
Sorption of water by spores, heat-killed spores, and vegetative cells |
title_full |
Sorption of water by spores, heat-killed spores, and vegetative cells |
title_fullStr |
Sorption of water by spores, heat-killed spores, and vegetative cells |
title_full_unstemmed |
Sorption of water by spores, heat-killed spores, and vegetative cells |
title_sort |
sorption of water by spores, heat-killed spores, and vegetative cells |
publisher |
Canadian Science Publishing |
publishDate |
1970 |
url |
http://dx.doi.org/10.1139/m70-096 http://www.nrcresearchpress.com/doi/pdf/10.1139/m70-096 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Canadian Journal of Microbiology volume 16, issue 7, page 573-579 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m70-096 |
container_title |
Canadian Journal of Microbiology |
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16 |
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7 |
container_start_page |
573 |
op_container_end_page |
579 |
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1785580062356013056 |