Immunological analysis of spores of Clostridium botulinum type E
High spore yields (>90%) of C. botulinum type E were obtained by a pseudosynchronous growth in trypticase peptone glucose broth. Clean spore suspensions of eight type E strains were prepared by enzymatic digestion of the vegetative components followed by differential centrifugation. Agglutina...
Published in: | Canadian Journal of Microbiology |
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Canadian Science Publishing
1969
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Online Access: | http://dx.doi.org/10.1139/m69-256 http://www.nrcresearchpress.com/doi/pdf/10.1139/m69-256 |
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crcansciencepubl:10.1139/m69-256 2023-12-17T10:28:02+01:00 Immunological analysis of spores of Clostridium botulinum type E Law, Simon W. T. Hawirko, R. Z. 1969 http://dx.doi.org/10.1139/m69-256 http://www.nrcresearchpress.com/doi/pdf/10.1139/m69-256 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 15, issue 12, page 1427-1431 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1969 crcansciencepubl https://doi.org/10.1139/m69-256 2023-11-19T13:38:30Z High spore yields (>90%) of C. botulinum type E were obtained by a pseudosynchronous growth in trypticase peptone glucose broth. Clean spore suspensions of eight type E strains were prepared by enzymatic digestion of the vegetative components followed by differential centrifugation. Agglutination tests of hyperimmune spore antiserum against type E 070 strain showed significant titers with spore suspensions of eight strains. Immunodiffusion tests with homologous antisera showed a multiplicity of antigens present in spore extracts of the Beluga and 070 strain. Some of the antigens appeared to be strain specific whereas others were shared by several strains. The precipitating antigens appeared to be type specific since precipitin lines did not form with spore extracts of C. botulinum types A and B. A line of identity was formed with spore extracts of seven toxigenic and one nontoxigenic strains, indicating the presence of a common spore antigen specific to type E strains. Article in Journal/Newspaper Beluga Beluga* Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 15 12 1427 1431 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Law, Simon W. T. Hawirko, R. Z. Immunological analysis of spores of Clostridium botulinum type E |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
High spore yields (>90%) of C. botulinum type E were obtained by a pseudosynchronous growth in trypticase peptone glucose broth. Clean spore suspensions of eight type E strains were prepared by enzymatic digestion of the vegetative components followed by differential centrifugation. Agglutination tests of hyperimmune spore antiserum against type E 070 strain showed significant titers with spore suspensions of eight strains. Immunodiffusion tests with homologous antisera showed a multiplicity of antigens present in spore extracts of the Beluga and 070 strain. Some of the antigens appeared to be strain specific whereas others were shared by several strains. The precipitating antigens appeared to be type specific since precipitin lines did not form with spore extracts of C. botulinum types A and B. A line of identity was formed with spore extracts of seven toxigenic and one nontoxigenic strains, indicating the presence of a common spore antigen specific to type E strains. |
format |
Article in Journal/Newspaper |
author |
Law, Simon W. T. Hawirko, R. Z. |
author_facet |
Law, Simon W. T. Hawirko, R. Z. |
author_sort |
Law, Simon W. T. |
title |
Immunological analysis of spores of Clostridium botulinum type E |
title_short |
Immunological analysis of spores of Clostridium botulinum type E |
title_full |
Immunological analysis of spores of Clostridium botulinum type E |
title_fullStr |
Immunological analysis of spores of Clostridium botulinum type E |
title_full_unstemmed |
Immunological analysis of spores of Clostridium botulinum type E |
title_sort |
immunological analysis of spores of clostridium botulinum type e |
publisher |
Canadian Science Publishing |
publishDate |
1969 |
url |
http://dx.doi.org/10.1139/m69-256 http://www.nrcresearchpress.com/doi/pdf/10.1139/m69-256 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
Canadian Journal of Microbiology volume 15, issue 12, page 1427-1431 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m69-256 |
container_title |
Canadian Journal of Microbiology |
container_volume |
15 |
container_issue |
12 |
container_start_page |
1427 |
op_container_end_page |
1431 |
_version_ |
1785580062153637888 |