SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL
A Debaryomyces kloeckeri strain from a Macquarie Island soil proved to be parathiotrophic, unable to satisfy its sulphur requirements from inorganic sources; certain sulphur amino acids were necessary. By contrast, inorganic sulphate and thiosulphate, but not dithionate, sulphide, or colloidal sulph...
Published in: | Canadian Journal of Microbiology |
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1964
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Online Access: | http://dx.doi.org/10.1139/m64-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/m64-053 |
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crcansciencepubl:10.1139/m64-053 2023-12-17T10:33:16+01:00 SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL Skerman, T. M. Singleton, R. J. 1964 http://dx.doi.org/10.1139/m64-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/m64-053 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 10, issue 3, page 397-406 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1964 crcansciencepubl https://doi.org/10.1139/m64-053 2023-11-19T13:39:00Z A Debaryomyces kloeckeri strain from a Macquarie Island soil proved to be parathiotrophic, unable to satisfy its sulphur requirements from inorganic sources; certain sulphur amino acids were necessary. By contrast, inorganic sulphate and thiosulphate, but not dithionate, sulphide, or colloidal sulphur, were effective sulphur sources for a second (euthiotrophic) strain.Both strains behaved identically towards various organic sulphur compounds tested as sole sulphur sources. Growth was supported by L-cysteine, L-cystine, DL-homocysteine, DL-cystathionine, L-cystathionine, DL-lanthionine, D- and L-methionine, methionine sulphoxide, methyl DL-methionine sulphonium chloride, and thiazolidine-4-carboxylic acid. Ineffective were methanethiol, thioglycollate, reduced glutathione, DL-homocystine, cysteic acid, taurine, methionine sulphone, djenkolic acid, thiamine, thiazole, thioacetamide, and thiourea. Biotin, though essential for both strains, was ineffective as a sole sulphur source.The parathiotrophic strain may be a mutant lacking an enzyme at some step in conversion of sulphate to cysteine. Because of difficulties with unstable intermediate compounds, the course of cysteine biosynthesis from sulphate in D. kloeckeri could not be defined from nutritional data alone, nor could one tell which of the sequential steps is blocked in the parathiotroph. Article in Journal/Newspaper Macquarie Island Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 10 3 397 406 |
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Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Skerman, T. M. Singleton, R. J. SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
A Debaryomyces kloeckeri strain from a Macquarie Island soil proved to be parathiotrophic, unable to satisfy its sulphur requirements from inorganic sources; certain sulphur amino acids were necessary. By contrast, inorganic sulphate and thiosulphate, but not dithionate, sulphide, or colloidal sulphur, were effective sulphur sources for a second (euthiotrophic) strain.Both strains behaved identically towards various organic sulphur compounds tested as sole sulphur sources. Growth was supported by L-cysteine, L-cystine, DL-homocysteine, DL-cystathionine, L-cystathionine, DL-lanthionine, D- and L-methionine, methionine sulphoxide, methyl DL-methionine sulphonium chloride, and thiazolidine-4-carboxylic acid. Ineffective were methanethiol, thioglycollate, reduced glutathione, DL-homocystine, cysteic acid, taurine, methionine sulphone, djenkolic acid, thiamine, thiazole, thioacetamide, and thiourea. Biotin, though essential for both strains, was ineffective as a sole sulphur source.The parathiotrophic strain may be a mutant lacking an enzyme at some step in conversion of sulphate to cysteine. Because of difficulties with unstable intermediate compounds, the course of cysteine biosynthesis from sulphate in D. kloeckeri could not be defined from nutritional data alone, nor could one tell which of the sequential steps is blocked in the parathiotroph. |
format |
Article in Journal/Newspaper |
author |
Skerman, T. M. Singleton, R. J. |
author_facet |
Skerman, T. M. Singleton, R. J. |
author_sort |
Skerman, T. M. |
title |
SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
title_short |
SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
title_full |
SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
title_fullStr |
SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
title_full_unstemmed |
SULPHUR NUTRITION OF TWO STRAINS OF THE YEAST, DEBARYOMYCES KLOECKERI, ISOLATED FROM SUBANTARCTIC SOIL |
title_sort |
sulphur nutrition of two strains of the yeast, debaryomyces kloeckeri, isolated from subantarctic soil |
publisher |
Canadian Science Publishing |
publishDate |
1964 |
url |
http://dx.doi.org/10.1139/m64-053 http://www.nrcresearchpress.com/doi/pdf/10.1139/m64-053 |
genre |
Macquarie Island |
genre_facet |
Macquarie Island |
op_source |
Canadian Journal of Microbiology volume 10, issue 3, page 397-406 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m64-053 |
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Canadian Journal of Microbiology |
container_volume |
10 |
container_issue |
3 |
container_start_page |
397 |
op_container_end_page |
406 |
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1785587213136822272 |