PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS
A number of Gram-negative cold-tolerant bacteria capable of growth on agar at 0 °C but not at 25 °C were isolated from Arctic littoral and marine sediment samples. Eight of these organisms able to hydrolyze gelatin were chosen for study. Taxonomically these organisms consisted of an unidentified but...
Published in: | Canadian Journal of Microbiology |
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Language: | English |
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Canadian Science Publishing
1963
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Online Access: | http://dx.doi.org/10.1139/m63-037 http://www.nrcresearchpress.com/doi/pdf/10.1139/m63-037 |
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crcansciencepubl:10.1139/m63-037 2023-12-17T10:24:17+01:00 PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS McDonald, I. J. Quadling, C. Chambers, Alice K. 1963 http://dx.doi.org/10.1139/m63-037 http://www.nrcresearchpress.com/doi/pdf/10.1139/m63-037 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 9, issue 3, page 303-315 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 1963 crcansciencepubl https://doi.org/10.1139/m63-037 2023-11-19T13:39:06Z A number of Gram-negative cold-tolerant bacteria capable of growth on agar at 0 °C but not at 25 °C were isolated from Arctic littoral and marine sediment samples. Eight of these organisms able to hydrolyze gelatin were chosen for study. Taxonomically these organisms consisted of an unidentified but closely related group of seven cultures and a single Pseudomonas strain. Culture supernatants of these Arctic organisms contained proteinases that hydrolyzed gelatin, casein, and β-lactoglobulin, but not bovine plasma albumin. When casein was the substrate, the proteinases were most active at pH 7.0 to 8.0 between 30° and 40 °C and were inhibited by ethylenediaminetetraacetic acid. The proteinases were relatively heat-labile; the activity of the most heat-labile was reduced by 90% in 20–30 minutes at 40 °C and that of the most heat-stable by 90% in 45 minutes at 50 °C. Proteinases of the unnamed group of Arctic organisms were characterized by a low temperature characteristic and possession of high enzymic activity at low temperatures. Presumably such organisms can convert protein to simple nitrogenous compounds in the Arctic environment. Article in Journal/Newspaper Arctic Canadian Science Publishing (via Crossref) Arctic Canadian Journal of Microbiology 9 3 303 315 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology McDonald, I. J. Quadling, C. Chambers, Alice K. PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
A number of Gram-negative cold-tolerant bacteria capable of growth on agar at 0 °C but not at 25 °C were isolated from Arctic littoral and marine sediment samples. Eight of these organisms able to hydrolyze gelatin were chosen for study. Taxonomically these organisms consisted of an unidentified but closely related group of seven cultures and a single Pseudomonas strain. Culture supernatants of these Arctic organisms contained proteinases that hydrolyzed gelatin, casein, and β-lactoglobulin, but not bovine plasma albumin. When casein was the substrate, the proteinases were most active at pH 7.0 to 8.0 between 30° and 40 °C and were inhibited by ethylenediaminetetraacetic acid. The proteinases were relatively heat-labile; the activity of the most heat-labile was reduced by 90% in 20–30 minutes at 40 °C and that of the most heat-stable by 90% in 45 minutes at 50 °C. Proteinases of the unnamed group of Arctic organisms were characterized by a low temperature characteristic and possession of high enzymic activity at low temperatures. Presumably such organisms can convert protein to simple nitrogenous compounds in the Arctic environment. |
format |
Article in Journal/Newspaper |
author |
McDonald, I. J. Quadling, C. Chambers, Alice K. |
author_facet |
McDonald, I. J. Quadling, C. Chambers, Alice K. |
author_sort |
McDonald, I. J. |
title |
PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
title_short |
PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
title_full |
PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
title_fullStr |
PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
title_full_unstemmed |
PROTEOLYTIC ACTIVITY OF SOME COLD-TOLERANT BACTERIA FROM ARCTIC SEDIMENTS |
title_sort |
proteolytic activity of some cold-tolerant bacteria from arctic sediments |
publisher |
Canadian Science Publishing |
publishDate |
1963 |
url |
http://dx.doi.org/10.1139/m63-037 http://www.nrcresearchpress.com/doi/pdf/10.1139/m63-037 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Canadian Journal of Microbiology volume 9, issue 3, page 303-315 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/m63-037 |
container_title |
Canadian Journal of Microbiology |
container_volume |
9 |
container_issue |
3 |
container_start_page |
303 |
op_container_end_page |
315 |
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1785564813105037312 |