Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotet...
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crcansciencepubl:10.1139/gen-2015-0014 2024-09-15T18:03:07+00:00 Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability Benabdelmouna, Abdellah Ledu, Christophe Hilliker, A.J. 2015 http://dx.doi.org/10.1139/gen-2015-0014 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2015-0014 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2015-0014 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 58, issue 7, page 333-348 ISSN 0831-2796 1480-3321 journal-article 2015 crcansciencepubl https://doi.org/10.1139/gen-2015-0014 2024-08-01T04:10:03Z We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested, showing that induction method has an important impact on the long-term cytogenetic stability of the tetraploids. It appears that induction method based on the use of triploid females induces a greater cytogenetic instability among tetraploids so obtained, and this compared to tetraploids originating from the two methods described in our present study. As the aneuploidies and reversions observed in tetraploids can have serious consequences for the sustainability of tetraploid broodstock itself, as well as their triploid offspring, the two tetraploid induction methods described in the present work offer means to produce tetraploids with optimal cytogenetic, genetic, and zootechnical performances. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Canadian Science Publishing Genome 58 7 333 348 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested, showing that induction method has an important impact on the long-term cytogenetic stability of the tetraploids. It appears that induction method based on the use of triploid females induces a greater cytogenetic instability among tetraploids so obtained, and this compared to tetraploids originating from the two methods described in our present study. As the aneuploidies and reversions observed in tetraploids can have serious consequences for the sustainability of tetraploid broodstock itself, as well as their triploid offspring, the two tetraploid induction methods described in the present work offer means to produce tetraploids with optimal cytogenetic, genetic, and zootechnical performances. |
author2 |
Hilliker, A.J. |
format |
Article in Journal/Newspaper |
author |
Benabdelmouna, Abdellah Ledu, Christophe |
spellingShingle |
Benabdelmouna, Abdellah Ledu, Christophe Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
author_facet |
Benabdelmouna, Abdellah Ledu, Christophe |
author_sort |
Benabdelmouna, Abdellah |
title |
Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_short |
Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_full |
Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_fullStr |
Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_full_unstemmed |
Autotetraploid Pacific oysters ( Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_sort |
autotetraploid pacific oysters ( crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
publisher |
Canadian Science Publishing |
publishDate |
2015 |
url |
http://dx.doi.org/10.1139/gen-2015-0014 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2015-0014 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2015-0014 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Genome volume 58, issue 7, page 333-348 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/gen-2015-0014 |
container_title |
Genome |
container_volume |
58 |
container_issue |
7 |
container_start_page |
333 |
op_container_end_page |
348 |
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1810440643954081792 |