Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus )
The mechanisms underlying sexual reproduction and sex ratio determination remains unclear in turbot, a flatfish of great commercial value. And there is limited information in the turbot database regarding genes related to the reproductive system. Here, we conducted high-throughput transcriptome prof...
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crcansciencepubl:10.1139/gen-2014-0190 2023-12-17T10:49:42+01:00 Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) Hu, Yulong Huang, Meng Wang, Weiji Guan, Jiantao Kong, Jie Danzmann, R. 2016 http://dx.doi.org/10.1139/gen-2014-0190 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2014-0190 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2014-0190 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 59, issue 1, page 1-10 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 2016 crcansciencepubl https://doi.org/10.1139/gen-2014-0190 2023-11-19T13:39:14Z The mechanisms underlying sexual reproduction and sex ratio determination remains unclear in turbot, a flatfish of great commercial value. And there is limited information in the turbot database regarding genes related to the reproductive system. Here, we conducted high-throughput transcriptome profiling of turbot gonad tissues to better understand their reproductive functions and to supply essential gene sequence information for marker-assisted selection programs in the turbot industry. In this study, two gonad libraries representing sex differences in Scophthalmus maximus yielded 453 818 high-quality reads that were assembled into 24 611 contigs and 33 713 singletons by using 454 pyrosequencing, 13 936 contigs and singletons (CS) of which were annotated using BLASTx. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses revealed that various biological functions and processes were associated with many of the annotated CS. Expression analyses showed that 510 genes were differentially expressed in males versus females; 80% of these genes were annotated. In addition, 6484 and 6036 single nucleotide polymorphisms (SNPs) were identified in male and female libraries, respectively. This transcriptome resource will serve as the foundation for cDNA or SNP microarray construction, gene expression characterization, and sex-specific linkage mapping in turbot. Article in Journal/Newspaper Scophthalmus maximus Turbot Canadian Science Publishing (via Crossref) Genome 59 1 1 10 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
spellingShingle |
Genetics Molecular Biology General Medicine Biotechnology Hu, Yulong Huang, Meng Wang, Weiji Guan, Jiantao Kong, Jie Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
The mechanisms underlying sexual reproduction and sex ratio determination remains unclear in turbot, a flatfish of great commercial value. And there is limited information in the turbot database regarding genes related to the reproductive system. Here, we conducted high-throughput transcriptome profiling of turbot gonad tissues to better understand their reproductive functions and to supply essential gene sequence information for marker-assisted selection programs in the turbot industry. In this study, two gonad libraries representing sex differences in Scophthalmus maximus yielded 453 818 high-quality reads that were assembled into 24 611 contigs and 33 713 singletons by using 454 pyrosequencing, 13 936 contigs and singletons (CS) of which were annotated using BLASTx. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses revealed that various biological functions and processes were associated with many of the annotated CS. Expression analyses showed that 510 genes were differentially expressed in males versus females; 80% of these genes were annotated. In addition, 6484 and 6036 single nucleotide polymorphisms (SNPs) were identified in male and female libraries, respectively. This transcriptome resource will serve as the foundation for cDNA or SNP microarray construction, gene expression characterization, and sex-specific linkage mapping in turbot. |
author2 |
Danzmann, R. |
format |
Article in Journal/Newspaper |
author |
Hu, Yulong Huang, Meng Wang, Weiji Guan, Jiantao Kong, Jie |
author_facet |
Hu, Yulong Huang, Meng Wang, Weiji Guan, Jiantao Kong, Jie |
author_sort |
Hu, Yulong |
title |
Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
title_short |
Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
title_full |
Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
title_fullStr |
Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
title_full_unstemmed |
Characterization of gonadal transcriptomes from the turbot ( Scophthalmus maximus ) |
title_sort |
characterization of gonadal transcriptomes from the turbot ( scophthalmus maximus ) |
publisher |
Canadian Science Publishing |
publishDate |
2016 |
url |
http://dx.doi.org/10.1139/gen-2014-0190 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2014-0190 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2014-0190 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Genome volume 59, issue 1, page 1-10 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/gen-2014-0190 |
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Genome |
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59 |
container_issue |
1 |
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1 |
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10 |
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1785574263903748096 |