The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens
Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding how elevated...
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crcansciencepubl:10.1139/gen-2013-0090 2024-06-23T07:51:02+00:00 The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens Hori, Tiago S. Gamperl, A. Kurt Nash, Gord Booman, Marije Barat, Ashoktaru Rise, Matthew L. Danzmann, R. 2013 http://dx.doi.org/10.1139/gen-2013-0090 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2013-0090 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2013-0090 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 56, issue 10, page 567-576 ISSN 0831-2796 1480-3321 journal-article 2013 crcansciencepubl https://doi.org/10.1139/gen-2013-0090 2024-05-30T08:13:50Z Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding how elevated temperatures impacts the immune response of this commercially important species may help to reduce the potential negative impacts of such challenges. Previously, we investigated the impacts of moderately elevated temperature on the antiviral responses of Atlantic cod (Hori et al. 2012) and reported that elevated temperature modulated the spleen transcriptome response to polyriboinosinic polyribocytidylic acid (pIC, a viral mimic). Herein, we report a complementary microarray study that investigated the impact of the same elevated temperature regime on the Atlantic cod spleen transcriptome response to intraperitoneal (IP) injection of formalin-killed Aeromonas salmonicida (ASAL). Fish were held at two different temperatures (10 °C and 16 °C) prior to immune stimulation and sampled 6 and 24 h post-injection (HPI). In this experiment, we identified 711 and 666 nonredundant ASAL-responsive genes at 6HPI and 24HPI, respectively. These included several known antibacterial genes, including hepcidin, cathelicidin, ferritin heavy subunit, and interleukin 8. However, we only identified 15 differentially expressed genes at 6HPI and 2 at 24HPI (FDR 1%) when comparing ASAL-injected fish held at 10 °C versus 16 °C. In contrast, the same comparisons with pIC-injected fish yielded 290 and 339 differentially expressed genes (FDR 1%) at 6HPI and 24HPI, respectively. These results suggest that moderately elevated temperature has a lesser effect on the Atlantic cod spleen transcriptome response to ASAL (i.e., the antibacterial response) than to pIC (i.e., antiviral response). Thus, the impacts of high temperatures on the cod’s immune response may be pathogen dependent. Article in Journal/Newspaper atlantic cod Gadus morhua Newfoundland Canadian Science Publishing Genome 56 10 567 576 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
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English |
description |
Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding how elevated temperatures impacts the immune response of this commercially important species may help to reduce the potential negative impacts of such challenges. Previously, we investigated the impacts of moderately elevated temperature on the antiviral responses of Atlantic cod (Hori et al. 2012) and reported that elevated temperature modulated the spleen transcriptome response to polyriboinosinic polyribocytidylic acid (pIC, a viral mimic). Herein, we report a complementary microarray study that investigated the impact of the same elevated temperature regime on the Atlantic cod spleen transcriptome response to intraperitoneal (IP) injection of formalin-killed Aeromonas salmonicida (ASAL). Fish were held at two different temperatures (10 °C and 16 °C) prior to immune stimulation and sampled 6 and 24 h post-injection (HPI). In this experiment, we identified 711 and 666 nonredundant ASAL-responsive genes at 6HPI and 24HPI, respectively. These included several known antibacterial genes, including hepcidin, cathelicidin, ferritin heavy subunit, and interleukin 8. However, we only identified 15 differentially expressed genes at 6HPI and 2 at 24HPI (FDR 1%) when comparing ASAL-injected fish held at 10 °C versus 16 °C. In contrast, the same comparisons with pIC-injected fish yielded 290 and 339 differentially expressed genes (FDR 1%) at 6HPI and 24HPI, respectively. These results suggest that moderately elevated temperature has a lesser effect on the Atlantic cod spleen transcriptome response to ASAL (i.e., the antibacterial response) than to pIC (i.e., antiviral response). Thus, the impacts of high temperatures on the cod’s immune response may be pathogen dependent. |
author2 |
Danzmann, R. |
format |
Article in Journal/Newspaper |
author |
Hori, Tiago S. Gamperl, A. Kurt Nash, Gord Booman, Marije Barat, Ashoktaru Rise, Matthew L. |
spellingShingle |
Hori, Tiago S. Gamperl, A. Kurt Nash, Gord Booman, Marije Barat, Ashoktaru Rise, Matthew L. The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
author_facet |
Hori, Tiago S. Gamperl, A. Kurt Nash, Gord Booman, Marije Barat, Ashoktaru Rise, Matthew L. |
author_sort |
Hori, Tiago S. |
title |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
title_short |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
title_full |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
title_fullStr |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
title_full_unstemmed |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod ( Gadus morhua) are stimulated with bacterial versus viral antigens |
title_sort |
impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether atlantic cod ( gadus morhua) are stimulated with bacterial versus viral antigens |
publisher |
Canadian Science Publishing |
publishDate |
2013 |
url |
http://dx.doi.org/10.1139/gen-2013-0090 http://www.nrcresearchpress.com/doi/full-xml/10.1139/gen-2013-0090 http://www.nrcresearchpress.com/doi/pdf/10.1139/gen-2013-0090 |
genre |
atlantic cod Gadus morhua Newfoundland |
genre_facet |
atlantic cod Gadus morhua Newfoundland |
op_source |
Genome volume 56, issue 10, page 567-576 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/gen-2013-0090 |
container_title |
Genome |
container_volume |
56 |
container_issue |
10 |
container_start_page |
567 |
op_container_end_page |
576 |
_version_ |
1802642030038351872 |