Homogenization of 5S ribosomal genes on the noncoding strand of the rDNA units of two crustacean species

• Published in: Genome
• Main Author: Drouin, Guy
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 1999
• Subjects: Genetics; Molecular Biology; General Medicine; Biotechnology; Calanus finmarchicus
• Online Access: http://dx.doi.org/10.1139/g98-113; http://www.nrcresearchpress.com/doi/pdf/10.1139/g98-113

We amplified and cloned twelve 28S-18S ribosomal RNA gene intergenic regions from two crustacean species, the copepod Calanus finmarchicus and the euphausid Thysanoessa raschi. Hybridization of single-stranded and double-stranded versions of these clones with single-stranded oligonucleotide probes showed that at least one 5S ribosomal gene is present on the noncoding strand of all twelve 28S-18S intergenic regions from each species.Key words: concerted evolution, 5S ribosomal genes, 18S ribosomal genes, 28S ribosomal genes.