Homogenization of 5S ribosomal genes on the noncoding strand of the rDNA units of two crustacean species

We amplified and cloned twelve 28S-18S ribosomal RNA gene intergenic regions from two crustacean species, the copepod Calanus finmarchicus and the euphausid Thysanoessa raschi. Hybridization of single-stranded and double-stranded versions of these clones with single-stranded oligonucleotide probes s...

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Bibliographic Details
Published in:Genome
Main Author: Drouin, Guy
Format: Article in Journal/Newspaper
Language:English
Published: Canadian Science Publishing 1999
Subjects:
Genetics
Molecular Biology
General Medicine
Biotechnology
Calanus finmarchicus
Online Access:http://dx.doi.org/10.1139/g98-113
http://www.nrcresearchpress.com/doi/pdf/10.1139/g98-113
Description
Summary:We amplified and cloned twelve 28S-18S ribosomal RNA gene intergenic regions from two crustacean species, the copepod Calanus finmarchicus and the euphausid Thysanoessa raschi. Hybridization of single-stranded and double-stranded versions of these clones with single-stranded oligonucleotide probes showed that at least one 5S ribosomal gene is present on the noncoding strand of all twelve 28S-18S intergenic regions from each species.Key words: concerted evolution, 5S ribosomal genes, 18S ribosomal genes, 28S ribosomal genes.

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