A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations
Almost 10% of the human genome consists of DNA sequences that share homology with retroviruses. These sequences, which represent a stable component of the human genome (although some may retain the ability to transpose), remain poorly understood. We used degenerate primers specific to the two conser...
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1998
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crcansciencepubl:10.1139/g98-072 2024-03-03T08:43:54+00:00 A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations Deb, Paromita Klempan, Timothy A O'Reilly, Richard L Singh, Shiva M 1998 http://dx.doi.org/10.1139/g98-072 http://www.nrcresearchpress.com/doi/pdf/10.1139/g98-072 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 41, issue 5, page 662-668 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 1998 crcansciencepubl https://doi.org/10.1139/g98-072 2024-02-07T10:53:33Z Almost 10% of the human genome consists of DNA sequences that share homology with retroviruses. These sequences, which represent a stable component of the human genome (although some may retain the ability to transpose), remain poorly understood. We used degenerate primers specific to the two conserved regions (boxes 4 and 5) of the retroviral pol gene, common to all retroviruses, and PCR-amplified related sequences from individuals representing two distinct populations: Caucasians and Dogrib Indians. The large number of sequences that are reproducibly amplified represent numerous sites of retroviral integration in the human genome. In both populations studied, one of the two primers yielded a polymorphic band, present in ~30% of the samples, that has probably been present in the human genome since before the divergence of the two populations ~10 000 years ago. It was established that this polymorphism was due to priming-site differences and not to deletions. Further, this priming site is duplicated at two genomic sites (representing 341- and 343-bp fragments) with at least two alleles each. Such novel polymorphisms should provide useful markers and permit assessment of evolutionary mechanisms associated with retroviral-related genomic evolution.Key words: Dogrib Indian, evolution, human genome, polymorphism, retrovirus. Article in Journal/Newspaper Dogrib Canadian Science Publishing Indian Genome 41 5 662 668 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
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Genetics Molecular Biology General Medicine Biotechnology Deb, Paromita Klempan, Timothy A O'Reilly, Richard L Singh, Shiva M A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
Almost 10% of the human genome consists of DNA sequences that share homology with retroviruses. These sequences, which represent a stable component of the human genome (although some may retain the ability to transpose), remain poorly understood. We used degenerate primers specific to the two conserved regions (boxes 4 and 5) of the retroviral pol gene, common to all retroviruses, and PCR-amplified related sequences from individuals representing two distinct populations: Caucasians and Dogrib Indians. The large number of sequences that are reproducibly amplified represent numerous sites of retroviral integration in the human genome. In both populations studied, one of the two primers yielded a polymorphic band, present in ~30% of the samples, that has probably been present in the human genome since before the divergence of the two populations ~10 000 years ago. It was established that this polymorphism was due to priming-site differences and not to deletions. Further, this priming site is duplicated at two genomic sites (representing 341- and 343-bp fragments) with at least two alleles each. Such novel polymorphisms should provide useful markers and permit assessment of evolutionary mechanisms associated with retroviral-related genomic evolution.Key words: Dogrib Indian, evolution, human genome, polymorphism, retrovirus. |
format |
Article in Journal/Newspaper |
author |
Deb, Paromita Klempan, Timothy A O'Reilly, Richard L Singh, Shiva M |
author_facet |
Deb, Paromita Klempan, Timothy A O'Reilly, Richard L Singh, Shiva M |
author_sort |
Deb, Paromita |
title |
A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
title_short |
A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
title_full |
A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
title_fullStr |
A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
title_full_unstemmed |
A single-primer PCR-based retroviral-related DNA polymorphism shared by two distinct human populations |
title_sort |
single-primer pcr-based retroviral-related dna polymorphism shared by two distinct human populations |
publisher |
Canadian Science Publishing |
publishDate |
1998 |
url |
http://dx.doi.org/10.1139/g98-072 http://www.nrcresearchpress.com/doi/pdf/10.1139/g98-072 |
geographic |
Indian |
geographic_facet |
Indian |
genre |
Dogrib |
genre_facet |
Dogrib |
op_source |
Genome volume 41, issue 5, page 662-668 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g98-072 |
container_title |
Genome |
container_volume |
41 |
container_issue |
5 |
container_start_page |
662 |
op_container_end_page |
668 |
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1792499376149495808 |