Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs)
Inter-Alu PCR is increasingly useful in human genome mapping studies. One use is the generation of alumorphs, polymorphisms resulting from the presence or absence of inter-Alu PCR products. In this study, we have increased the proportion of the genome that can be analyzed by this technique with the...
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1996
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crcansciencepubl:10.1139/g96-087 2023-12-17T10:29:44+01:00 Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) Kass, David H. Batzer, Mark A. Deininger, Prescott L. 1996 http://dx.doi.org/10.1139/g96-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/g96-087 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 39, issue 4, page 688-696 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 1996 crcansciencepubl https://doi.org/10.1139/g96-087 2023-11-19T13:39:15Z Inter-Alu PCR is increasingly useful in human genome mapping studies. One use is the generation of alumorphs, polymorphisms resulting from the presence or absence of inter-Alu PCR products. In this study, we have increased the proportion of the genome that can be analyzed by this technique with the use of long interspersed elements (LINEs). The set of polymorphisms detected by both Alu and LINE primers are referred to as interspersed repetitive sequence variants or IRS-morphs. Since a presence–absence variant may have been the result of a recent Alu or LINE insertion, we analyzed 7 isolated IRS-morphs that were generated, in part, with a primer derived from either a consensus LINE or a young Alu subfamily specific sequence, and observed by Southern blot analysis that these variants resulted from other types of genomic alterations. The use of these primers, however, reduces background from the numerous LINEs and Alu elements in the genome, providing sharp DNA fingerprint profiles. We have demonstrated the potential usefulness of these IRS-morph profiles in human population studies. We compared 12 IRS-morphs from a single amplification reaction from five distinct population groups (Caucasian (northern European descent), Hispanic (Mexican-American), Hindu-Indian, Papua New Guinean, and Greenland Eskimo) and observed that most have variable allelic frequencies among populations. The utilization of additional IRS-morph profiles will perpetuate this technique as a tool for DNA fingerprinting and for the analysis of human populations. Key words : Alu elements, DNA fingerprint, human populations, LINEs, SINEs. Article in Journal/Newspaper eskimo* Greenland Canadian Science Publishing (via Crossref) Greenland Indian Genome 39 4 688 696 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
spellingShingle |
Genetics Molecular Biology General Medicine Biotechnology Kass, David H. Batzer, Mark A. Deininger, Prescott L. Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
Inter-Alu PCR is increasingly useful in human genome mapping studies. One use is the generation of alumorphs, polymorphisms resulting from the presence or absence of inter-Alu PCR products. In this study, we have increased the proportion of the genome that can be analyzed by this technique with the use of long interspersed elements (LINEs). The set of polymorphisms detected by both Alu and LINE primers are referred to as interspersed repetitive sequence variants or IRS-morphs. Since a presence–absence variant may have been the result of a recent Alu or LINE insertion, we analyzed 7 isolated IRS-morphs that were generated, in part, with a primer derived from either a consensus LINE or a young Alu subfamily specific sequence, and observed by Southern blot analysis that these variants resulted from other types of genomic alterations. The use of these primers, however, reduces background from the numerous LINEs and Alu elements in the genome, providing sharp DNA fingerprint profiles. We have demonstrated the potential usefulness of these IRS-morph profiles in human population studies. We compared 12 IRS-morphs from a single amplification reaction from five distinct population groups (Caucasian (northern European descent), Hispanic (Mexican-American), Hindu-Indian, Papua New Guinean, and Greenland Eskimo) and observed that most have variable allelic frequencies among populations. The utilization of additional IRS-morph profiles will perpetuate this technique as a tool for DNA fingerprinting and for the analysis of human populations. Key words : Alu elements, DNA fingerprint, human populations, LINEs, SINEs. |
format |
Article in Journal/Newspaper |
author |
Kass, David H. Batzer, Mark A. Deininger, Prescott L. |
author_facet |
Kass, David H. Batzer, Mark A. Deininger, Prescott L. |
author_sort |
Kass, David H. |
title |
Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
title_short |
Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
title_full |
Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
title_fullStr |
Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
title_full_unstemmed |
Characterization and population diversity of interspersed repeat sequence variants (IRS-morphs) |
title_sort |
characterization and population diversity of interspersed repeat sequence variants (irs-morphs) |
publisher |
Canadian Science Publishing |
publishDate |
1996 |
url |
http://dx.doi.org/10.1139/g96-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/g96-087 |
geographic |
Greenland Indian |
geographic_facet |
Greenland Indian |
genre |
eskimo* Greenland |
genre_facet |
eskimo* Greenland |
op_source |
Genome volume 39, issue 4, page 688-696 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g96-087 |
container_title |
Genome |
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39 |
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4 |
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688 |
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696 |
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1785582503799554048 |