Characterization of a repetitive element detected by Nhe I in the genomes of Salmo species

• Published in: Genome
• Main Authors: Goodier, John L., Davidson, William S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 1994
• Subjects: Genetics; Molecular Biology; General Medicine; Biotechnology; Atlantic salmon
• Online Access: http://dx.doi.org/10.1139/g94-091; http://www.nrcresearchpress.com/doi/pdf/10.1139/g94-091

The genomes of the two species in the genus Salmo (Atlantic salmon, S. salar; brown trout, S. trutta) contain a 380-bp repetitive element that is flanked by the recognition sequence of the restriction enzyme NheI. These elements, which comprise approximately 1.2% of the salmon genome, do not exist in long tandem arrays as is typical of satellite DNA. A comparison of the sequences of 16 salmon and 7 trout elements revealed that members of this family of repetitive DNA are closely related to one another (over 95% identity). Subfamily structure exists and there is evidence that members of the same subfamilies are found in both Salmo species. A search of the GenBank database indicated that sequences homologous to the NheI repeat are located within a 1424-bp segment inserted immediately downstream of the 5′ end of a Tc1 transposon-like sequence isolated from Atlantic salmon (A.R. Radice, B. Bugaj, D.H. Fitch, and S.W. Emmons, unpublished data; GenBank accession No. L12206).Key words: satellite DNA, Atlantic salmon, brown trout, Tc1-like transposon, evolution.