Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae)
A cDNA encoding triose phosphate isomerase (TPI) of Stellaria longipes has been isolated and sequenced. The TPI of S. longipes exhibits high similarity to the TPIs from other species at both the nucleotide and amino acid levels. Southern blot analysis showed that the S. longipes genome contained mul...
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Canadian Science Publishing
1994
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Online Access: | http://dx.doi.org/10.1139/g94-019 http://www.nrcresearchpress.com/doi/pdf/10.1139/g94-019 |
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crcansciencepubl:10.1139/g94-019 2023-12-17T10:50:42+01:00 Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) Zhang, Xing-Hai Chinnappa, C. C. 1994 http://dx.doi.org/10.1139/g94-019 http://www.nrcresearchpress.com/doi/pdf/10.1139/g94-019 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 37, issue 1, page 148-156 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 1994 crcansciencepubl https://doi.org/10.1139/g94-019 2023-11-19T13:38:32Z A cDNA encoding triose phosphate isomerase (TPI) of Stellaria longipes has been isolated and sequenced. The TPI of S. longipes exhibits high similarity to the TPIs from other species at both the nucleotide and amino acid levels. Southern blot analysis showed that the S. longipes genome contained multiple TPI-like nucleotide sequences and only a low degree of polymorphism was observed among genotypes of different habitats. The levels of TPI mRNA, detected by hybridization to the TPI cDNA, appeared similar between the genotypes. However, different genotypes showed variations in the total TPI activity, reflecting the possible ecological effect on the TPI gene expression. Northern blot hybridization to the cDNA also indicated a higher level of TPI mRNA in leaves than in roots, suggesting tissue-specific expression of TPI gene(s). Phylogenetic analysis of the TPI amino acid sequences from 16 taxa suggested that the TPI from S. longipes was more closely related to cytosolic TPIs from other plants than it was to TPIs from prokaryotes.Key words: triose phosphate isomerase, Stellaria longipes, genotype, ecotypes, molecular phylogeny. Article in Journal/Newspaper Stellaria longipes Canadian Science Publishing (via Crossref) Genome 37 1 148 156 |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
spellingShingle |
Genetics Molecular Biology General Medicine Biotechnology Zhang, Xing-Hai Chinnappa, C. C. Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
A cDNA encoding triose phosphate isomerase (TPI) of Stellaria longipes has been isolated and sequenced. The TPI of S. longipes exhibits high similarity to the TPIs from other species at both the nucleotide and amino acid levels. Southern blot analysis showed that the S. longipes genome contained multiple TPI-like nucleotide sequences and only a low degree of polymorphism was observed among genotypes of different habitats. The levels of TPI mRNA, detected by hybridization to the TPI cDNA, appeared similar between the genotypes. However, different genotypes showed variations in the total TPI activity, reflecting the possible ecological effect on the TPI gene expression. Northern blot hybridization to the cDNA also indicated a higher level of TPI mRNA in leaves than in roots, suggesting tissue-specific expression of TPI gene(s). Phylogenetic analysis of the TPI amino acid sequences from 16 taxa suggested that the TPI from S. longipes was more closely related to cytosolic TPIs from other plants than it was to TPIs from prokaryotes.Key words: triose phosphate isomerase, Stellaria longipes, genotype, ecotypes, molecular phylogeny. |
format |
Article in Journal/Newspaper |
author |
Zhang, Xing-Hai Chinnappa, C. C. |
author_facet |
Zhang, Xing-Hai Chinnappa, C. C. |
author_sort |
Zhang, Xing-Hai |
title |
Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
title_short |
Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
title_full |
Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
title_fullStr |
Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
title_full_unstemmed |
Triose phosphate isomerase of Stellaria longipes (Caryophyllaceae) |
title_sort |
triose phosphate isomerase of stellaria longipes (caryophyllaceae) |
publisher |
Canadian Science Publishing |
publishDate |
1994 |
url |
http://dx.doi.org/10.1139/g94-019 http://www.nrcresearchpress.com/doi/pdf/10.1139/g94-019 |
genre |
Stellaria longipes |
genre_facet |
Stellaria longipes |
op_source |
Genome volume 37, issue 1, page 148-156 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g94-019 |
container_title |
Genome |
container_volume |
37 |
container_issue |
1 |
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148 |
op_container_end_page |
156 |
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1785575745599307776 |