Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of d...
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crcansciencepubl:10.1139/g04-087 2023-12-17T10:29:02+01:00 Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas Bouilly, Karine Leitão, Alexandra Chaves, Raquel Guedes-Pinto, Henrique Boudry, Pierre Lapègue, Sylvie 2005 http://dx.doi.org/10.1139/g04-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/g04-087 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 48, issue 1, page 177-180 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 2005 crcansciencepubl https://doi.org/10.1139/g04-087 2023-11-19T13:38:40Z Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were analysed using the restriction enzyme HaeIII. The study of 26 RE-banded aneuploid karyotypes showed that the same chromosome pairs (1, 5, 9, and 10) were affected by the loss of 1 chromosome (61%, 15%, 42%, and 42%, respectively). Further investigation is required to enable a better understanding of aneuploidy in oysters, especially with respect to why some chromosomes are more easily lost than others, and why cells tolerate the loss of these chromosomes.Key words: aneuploidy, atrazine, restriction enzyme digestion chromosome banding, Pacific oyster, Crassostrea gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Canadian Science Publishing (via Crossref) Pacific Genome 48 1 177 180 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
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Genetics Molecular Biology General Medicine Biotechnology Bouilly, Karine Leitão, Alexandra Chaves, Raquel Guedes-Pinto, Henrique Boudry, Pierre Lapègue, Sylvie Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were analysed using the restriction enzyme HaeIII. The study of 26 RE-banded aneuploid karyotypes showed that the same chromosome pairs (1, 5, 9, and 10) were affected by the loss of 1 chromosome (61%, 15%, 42%, and 42%, respectively). Further investigation is required to enable a better understanding of aneuploidy in oysters, especially with respect to why some chromosomes are more easily lost than others, and why cells tolerate the loss of these chromosomes.Key words: aneuploidy, atrazine, restriction enzyme digestion chromosome banding, Pacific oyster, Crassostrea gigas. |
format |
Article in Journal/Newspaper |
author |
Bouilly, Karine Leitão, Alexandra Chaves, Raquel Guedes-Pinto, Henrique Boudry, Pierre Lapègue, Sylvie |
author_facet |
Bouilly, Karine Leitão, Alexandra Chaves, Raquel Guedes-Pinto, Henrique Boudry, Pierre Lapègue, Sylvie |
author_sort |
Bouilly, Karine |
title |
Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
title_short |
Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
title_full |
Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
title_fullStr |
Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
title_full_unstemmed |
Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas |
title_sort |
endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in crassostrea gigas |
publisher |
Canadian Science Publishing |
publishDate |
2005 |
url |
http://dx.doi.org/10.1139/g04-087 http://www.nrcresearchpress.com/doi/pdf/10.1139/g04-087 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Genome volume 48, issue 1, page 177-180 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g04-087 |
container_title |
Genome |
container_volume |
48 |
container_issue |
1 |
container_start_page |
177 |
op_container_end_page |
180 |
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1785581292950126592 |