Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae
Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, st...
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crcansciencepubl:10.1139/g04-035 2023-12-17T10:29:12+01:00 Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae Leitão, A Chaves, R Santos, S Guedes-Pinto, H Boudry, P 2004 http://dx.doi.org/10.1139/g04-035 http://www.nrcresearchpress.com/doi/pdf/10.1139/g04-035 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 47, issue 5, page 781-788 ISSN 0831-2796 1480-3321 Genetics Molecular Biology General Medicine Biotechnology journal-article 2004 crcansciencepubl https://doi.org/10.1139/g04-035 2023-11-19T13:39:10Z Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassostrea gigas (2n = 20; total number of bands with ApaI, 74; HaeIII, 61; PstI, 76), Crassostrea angulata (2n = 20; ApaI, 62; HaeIII, 61; PstI, 55) (subfamily Crassostreinae), Ostrea edulis (2n = 20; ApaI, 82; HaeIII, 59; PstI, 66), and Ostrea conchaphila (2n = 20; ApaI, 68; HaeIII, 62; PstI, 69) (subfamily Ostreinae). Treatment of samples with ApaI, HaeIII, and PstI produced specific banding patterns, which demonstrates the potential of these enzymes for chromosome banding in oysters. This is of special interest, since it has been recently shown in mammalian chromosomes that restriction enzyme banding is compatible with fluorescence in situ hybridization. This study therefore provides a fundamental step in genome mapping of oysters, since chromosome banding with restriction enzymes facilitates physical gene mapping in these important aquaculture species. The analysis of the banded karyotypes revealed a greater similarity within the genera of Crassostrea and Ostrea than between them.Key words: Ostreidae, Crassostrea, Ostrea, chromosome banding, in situ restriction enzyme banding. Article in Journal/Newspaper Crassostrea gigas Canadian Science Publishing (via Crossref) Genome 47 5 781 788 |
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Canadian Science Publishing (via Crossref) |
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English |
topic |
Genetics Molecular Biology General Medicine Biotechnology |
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Genetics Molecular Biology General Medicine Biotechnology Leitão, A Chaves, R Santos, S Guedes-Pinto, H Boudry, P Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
topic_facet |
Genetics Molecular Biology General Medicine Biotechnology |
description |
Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassostrea gigas (2n = 20; total number of bands with ApaI, 74; HaeIII, 61; PstI, 76), Crassostrea angulata (2n = 20; ApaI, 62; HaeIII, 61; PstI, 55) (subfamily Crassostreinae), Ostrea edulis (2n = 20; ApaI, 82; HaeIII, 59; PstI, 66), and Ostrea conchaphila (2n = 20; ApaI, 68; HaeIII, 62; PstI, 69) (subfamily Ostreinae). Treatment of samples with ApaI, HaeIII, and PstI produced specific banding patterns, which demonstrates the potential of these enzymes for chromosome banding in oysters. This is of special interest, since it has been recently shown in mammalian chromosomes that restriction enzyme banding is compatible with fluorescence in situ hybridization. This study therefore provides a fundamental step in genome mapping of oysters, since chromosome banding with restriction enzymes facilitates physical gene mapping in these important aquaculture species. The analysis of the banded karyotypes revealed a greater similarity within the genera of Crassostrea and Ostrea than between them.Key words: Ostreidae, Crassostrea, Ostrea, chromosome banding, in situ restriction enzyme banding. |
format |
Article in Journal/Newspaper |
author |
Leitão, A Chaves, R Santos, S Guedes-Pinto, H Boudry, P |
author_facet |
Leitão, A Chaves, R Santos, S Guedes-Pinto, H Boudry, P |
author_sort |
Leitão, A |
title |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_short |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_full |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_fullStr |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_full_unstemmed |
Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae |
title_sort |
restriction enzyme digestion chromosome banding in crassostrea and ostrea species: comparative karyological analysis within ostreidae |
publisher |
Canadian Science Publishing |
publishDate |
2004 |
url |
http://dx.doi.org/10.1139/g04-035 http://www.nrcresearchpress.com/doi/pdf/10.1139/g04-035 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Genome volume 47, issue 5, page 781-788 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g04-035 |
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Genome |
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47 |
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5 |
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781 |
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788 |
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1785581529054838784 |