Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex
Genetic variation in the allotetraploid grass Elymus alaskanus complex was assessed using microsatellites in seven populations from Canada, Greenland, and the U.S.A. Microsatellite variation was compared with allozyme and RAPD variation. Our results indicated that E. alaskanus was highly homozygous...
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crcansciencepubl:10.1139/g03-052 2024-09-15T18:09:24+00:00 Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex Sun, Genlou Salomon, Björn 2003 http://dx.doi.org/10.1139/g03-052 http://www.nrcresearchpress.com/doi/pdf/10.1139/g03-052 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 46, issue 5, page 729-737 ISSN 0831-2796 1480-3321 journal-article 2003 crcansciencepubl https://doi.org/10.1139/g03-052 2024-07-11T04:12:01Z Genetic variation in the allotetraploid grass Elymus alaskanus complex was assessed using microsatellites in seven populations from Canada, Greenland, and the U.S.A. Microsatellite variation was compared with allozyme and RAPD variation. Our results indicated that E. alaskanus was highly homozygous but also highly variable. The polymorphic loci ranged from 50 to 100% with a mean of 78.6%, and the mean number of allele per locus was 3.14. Average expected heterozygosity value (H E , gene diversity) varied across populations and ranged from 0.244 to 0.651 with mean of 0.414. The mean value of H E across Canadian populations (0.517) was significantly higher than that across populations in Greenland (0.367). The correlation between allozyme and microsatellite gene diversity value (H E ) showed a high positive correlation (r = 0.68), but between RAPD and microsatellite showed a low positive correlation (r = 0.08). Populations were highly differentiated, with 38% of variation among populations. Interpopulation genetic distance showed no association with geographic distance between the population sites of origin. A Hardy-Weinberg exact test for all loci and all populations reveals a significant heterozygote deficiency. Possible explanations for heterozygote deficiency are discussed.Key words: Elymus alaskanus, microsatellites, heterozygote deficiency, genetic differentiation, variability. Article in Journal/Newspaper Greenland Canadian Science Publishing Genome 46 5 729 737 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
Genetic variation in the allotetraploid grass Elymus alaskanus complex was assessed using microsatellites in seven populations from Canada, Greenland, and the U.S.A. Microsatellite variation was compared with allozyme and RAPD variation. Our results indicated that E. alaskanus was highly homozygous but also highly variable. The polymorphic loci ranged from 50 to 100% with a mean of 78.6%, and the mean number of allele per locus was 3.14. Average expected heterozygosity value (H E , gene diversity) varied across populations and ranged from 0.244 to 0.651 with mean of 0.414. The mean value of H E across Canadian populations (0.517) was significantly higher than that across populations in Greenland (0.367). The correlation between allozyme and microsatellite gene diversity value (H E ) showed a high positive correlation (r = 0.68), but between RAPD and microsatellite showed a low positive correlation (r = 0.08). Populations were highly differentiated, with 38% of variation among populations. Interpopulation genetic distance showed no association with geographic distance between the population sites of origin. A Hardy-Weinberg exact test for all loci and all populations reveals a significant heterozygote deficiency. Possible explanations for heterozygote deficiency are discussed.Key words: Elymus alaskanus, microsatellites, heterozygote deficiency, genetic differentiation, variability. |
format |
Article in Journal/Newspaper |
author |
Sun, Genlou Salomon, Björn |
spellingShingle |
Sun, Genlou Salomon, Björn Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
author_facet |
Sun, Genlou Salomon, Björn |
author_sort |
Sun, Genlou |
title |
Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
title_short |
Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
title_full |
Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
title_fullStr |
Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
title_full_unstemmed |
Microsatellite variability and heterozygote deficiency in the arcticalpine Alaskan wheatgrass ( Elymusalaskanus) complex |
title_sort |
microsatellite variability and heterozygote deficiency in the arcticalpine alaskan wheatgrass ( elymusalaskanus) complex |
publisher |
Canadian Science Publishing |
publishDate |
2003 |
url |
http://dx.doi.org/10.1139/g03-052 http://www.nrcresearchpress.com/doi/pdf/10.1139/g03-052 |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Genome volume 46, issue 5, page 729-737 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g03-052 |
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Genome |
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46 |
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5 |
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729 |
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737 |
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1810446946865774592 |