Characterization of the red knot ( Calidris canutus) mitochondrial control region
We sequenced the complete mitochondrial control regions of 11 red knots (Calidris canutus). The control region is 1168 bp in length and is flanked by tRNA glutamate (glu) and the gene ND6 at its 5' end and tRNA phenylalanine (phe) and the gene 12S on its 3' end. The sequence possesses cons...
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2003
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Online Access: | http://dx.doi.org/10.1139/g03-034 http://www.nrcresearchpress.com/doi/pdf/10.1139/g03-034 |
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crcansciencepubl:10.1139/g03-034 2024-06-23T07:51:55+00:00 Characterization of the red knot ( Calidris canutus) mitochondrial control region Buehler, Deborah M Baker, Allan J 2003 http://dx.doi.org/10.1139/g03-034 http://www.nrcresearchpress.com/doi/pdf/10.1139/g03-034 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Genome volume 46, issue 4, page 565-572 ISSN 0831-2796 1480-3321 journal-article 2003 crcansciencepubl https://doi.org/10.1139/g03-034 2024-05-24T13:05:50Z We sequenced the complete mitochondrial control regions of 11 red knots (Calidris canutus). The control region is 1168 bp in length and is flanked by tRNA glutamate (glu) and the gene ND6 at its 5' end and tRNA phenylalanine (phe) and the gene 12S on its 3' end. The sequence possesses conserved sequence blocks F, E, D, C, CSB-1, and the bird similarity box (BSB), as expected for a mitochondrial copy. Flanking tRNA regions show correct secondary structure, and a relative rate test indicated no significant difference between substitution rates in the sequence we obtained versus the known mitochondrial sequence of turnstones (Charadriiformes: Scolopacidae). These characteristics indicate that the sequence is mitochondrial in origin. To confirm this, we sequenced the control region of a single individual using both purified mitochondrial DNA and genomic DNA. The sequences were identical using both methods. The sequence and methods presented in this paper may now serve as a reference for future studies using knot and other avian control regions. Furthermore, the discovery of five variable sites in 11 knots towards the 3' end of the control region, and the variability of this region in contrast to the more conserved central domain in the alignment between knots and other Charadriiformes, highlights the importance of this area as a source of variation for future studies in knots and other birds.Key words: D-loop, Calidris canutus, Charadriiformes, Aves, evolution. Article in Journal/Newspaper Calidris canutus Red Knot Canadian Science Publishing Genome 46 4 565 572 |
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Open Polar |
collection |
Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
We sequenced the complete mitochondrial control regions of 11 red knots (Calidris canutus). The control region is 1168 bp in length and is flanked by tRNA glutamate (glu) and the gene ND6 at its 5' end and tRNA phenylalanine (phe) and the gene 12S on its 3' end. The sequence possesses conserved sequence blocks F, E, D, C, CSB-1, and the bird similarity box (BSB), as expected for a mitochondrial copy. Flanking tRNA regions show correct secondary structure, and a relative rate test indicated no significant difference between substitution rates in the sequence we obtained versus the known mitochondrial sequence of turnstones (Charadriiformes: Scolopacidae). These characteristics indicate that the sequence is mitochondrial in origin. To confirm this, we sequenced the control region of a single individual using both purified mitochondrial DNA and genomic DNA. The sequences were identical using both methods. The sequence and methods presented in this paper may now serve as a reference for future studies using knot and other avian control regions. Furthermore, the discovery of five variable sites in 11 knots towards the 3' end of the control region, and the variability of this region in contrast to the more conserved central domain in the alignment between knots and other Charadriiformes, highlights the importance of this area as a source of variation for future studies in knots and other birds.Key words: D-loop, Calidris canutus, Charadriiformes, Aves, evolution. |
format |
Article in Journal/Newspaper |
author |
Buehler, Deborah M Baker, Allan J |
spellingShingle |
Buehler, Deborah M Baker, Allan J Characterization of the red knot ( Calidris canutus) mitochondrial control region |
author_facet |
Buehler, Deborah M Baker, Allan J |
author_sort |
Buehler, Deborah M |
title |
Characterization of the red knot ( Calidris canutus) mitochondrial control region |
title_short |
Characterization of the red knot ( Calidris canutus) mitochondrial control region |
title_full |
Characterization of the red knot ( Calidris canutus) mitochondrial control region |
title_fullStr |
Characterization of the red knot ( Calidris canutus) mitochondrial control region |
title_full_unstemmed |
Characterization of the red knot ( Calidris canutus) mitochondrial control region |
title_sort |
characterization of the red knot ( calidris canutus) mitochondrial control region |
publisher |
Canadian Science Publishing |
publishDate |
2003 |
url |
http://dx.doi.org/10.1139/g03-034 http://www.nrcresearchpress.com/doi/pdf/10.1139/g03-034 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Genome volume 46, issue 4, page 565-572 ISSN 0831-2796 1480-3321 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/g03-034 |
container_title |
Genome |
container_volume |
46 |
container_issue |
4 |
container_start_page |
565 |
op_container_end_page |
572 |
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1802643067203747840 |