Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis

• Published in: Genome
• Main Authors: Lindner, K R, Seeb, J E, Habicht, C, Knudsen, K L, Kretschmer, E, Reedy, D J, Spruell, P, Allendorf, F W
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 2000
• Subjects: Oncorhynchus gorbuscha; Pink salmon
• Online Access: http://dx.doi.org/10.1139/g00-016; http://www.nrcresearchpress.com/doi/pdf/10.1139/g00-016

We estimated recombination rates between 312 loci and their centromeres in gynogenetic diploid pink salmon (Oncorhynchus gorbuscha) that we produced by initiating development with irradiated sperm and blocking the maternal second meiotic division. Amplified fragment length polymorphisms (AFLPs) were significantly more centromeric than loci identified by three other techniques (allozymes, microsatellites, and PCR using primer sequences from interspersed nuclear elements). The near absence of AFLPs in distal regions could limit their utility in constructing linkage maps. A large proportion of loci had frequency of second division segregation (y) values approaching 1.0, indicating near complete crossover interference on many chromosome arms. As predicted from models of chromosomal evolution in salmonids based upon results with allozyme loci, all duplicated microsatellite loci that shared alleles (isoloci) had y values of nearly 1.0.Key words: meiosis, AFLP, microsatellites, isoloci, Oncorhynchus gorbuscha.