Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae)
Two highly polymorphic microsatellite DNA loci were used to determine paternity of larvae of primiparous Chionoecetes opilio females. In addition, female spermathecal contents were genotyped at the two loci and histological analysis of the organs was carried out to elucidate patterns of sperm compet...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Language: | English |
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Canadian Science Publishing
1998
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Online Access: | http://dx.doi.org/10.1139/f98-006 http://www.nrcresearchpress.com/doi/pdf/10.1139/f98-006 |
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crcansciencepubl:10.1139/f98-006 2024-09-15T18:01:54+00:00 Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) Urbani, Nicola Sainte-Marie, Bernard Sévigny, Jean-Marie Zadworny, David Kuhnlein, Urs 1998 http://dx.doi.org/10.1139/f98-006 http://www.nrcresearchpress.com/doi/pdf/10.1139/f98-006 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 55, issue 5, page 1104-1113 ISSN 0706-652X 1205-7533 journal-article 1998 crcansciencepubl https://doi.org/10.1139/f98-006 2024-08-08T04:13:36Z Two highly polymorphic microsatellite DNA loci were used to determine paternity of larvae of primiparous Chionoecetes opilio females. In addition, female spermathecal contents were genotyped at the two loci and histological analysis of the organs was carried out to elucidate patterns of sperm competition. Females carrying their first brood from controlled laboratory matings and from matings in the wild were examined. Spermathecal contents from wild-caught females were cut into several cross sections and each section genotyped individually. Both wild and laboratory females commonly mated with several males whose ejaculates were stored and stratified in the spermathecae. Genetic typing of the offspring of laboratory-mated females revealed single paternity and indicated that the last mate to inseminate a female before oviposition gained paternity of the clutch. The predominant mechanism ensuring single paternity appeared to be sperm stratification. In wild-caught females, the microsatellite typing of the offspring also revealed single paternity, but larvae appeared to be sired by males whose genotypes were found in the spermathecal cross sections towards the dorsal end (blind end) of the spermathecae. This suggested that they were the first males to mate with females that they guarded until oviposition, and females remated with other males thereafter. Article in Journal/Newspaper Chionoecetes opilio Snow crab Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 55 5 1104 1113 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
Two highly polymorphic microsatellite DNA loci were used to determine paternity of larvae of primiparous Chionoecetes opilio females. In addition, female spermathecal contents were genotyped at the two loci and histological analysis of the organs was carried out to elucidate patterns of sperm competition. Females carrying their first brood from controlled laboratory matings and from matings in the wild were examined. Spermathecal contents from wild-caught females were cut into several cross sections and each section genotyped individually. Both wild and laboratory females commonly mated with several males whose ejaculates were stored and stratified in the spermathecae. Genetic typing of the offspring of laboratory-mated females revealed single paternity and indicated that the last mate to inseminate a female before oviposition gained paternity of the clutch. The predominant mechanism ensuring single paternity appeared to be sperm stratification. In wild-caught females, the microsatellite typing of the offspring also revealed single paternity, but larvae appeared to be sired by males whose genotypes were found in the spermathecal cross sections towards the dorsal end (blind end) of the spermathecae. This suggested that they were the first males to mate with females that they guarded until oviposition, and females remated with other males thereafter. |
format |
Article in Journal/Newspaper |
author |
Urbani, Nicola Sainte-Marie, Bernard Sévigny, Jean-Marie Zadworny, David Kuhnlein, Urs |
spellingShingle |
Urbani, Nicola Sainte-Marie, Bernard Sévigny, Jean-Marie Zadworny, David Kuhnlein, Urs Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
author_facet |
Urbani, Nicola Sainte-Marie, Bernard Sévigny, Jean-Marie Zadworny, David Kuhnlein, Urs |
author_sort |
Urbani, Nicola |
title |
Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
title_short |
Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
title_full |
Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
title_fullStr |
Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
title_full_unstemmed |
Sperm competition and paternity assurance during the first breeding period of female snow crab ( Chionoecetes opilio) (Brachyura: Majidae) |
title_sort |
sperm competition and paternity assurance during the first breeding period of female snow crab ( chionoecetes opilio) (brachyura: majidae) |
publisher |
Canadian Science Publishing |
publishDate |
1998 |
url |
http://dx.doi.org/10.1139/f98-006 http://www.nrcresearchpress.com/doi/pdf/10.1139/f98-006 |
genre |
Chionoecetes opilio Snow crab |
genre_facet |
Chionoecetes opilio Snow crab |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 55, issue 5, page 1104-1113 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f98-006 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
55 |
container_issue |
5 |
container_start_page |
1104 |
op_container_end_page |
1113 |
_version_ |
1810438963215728640 |