Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations
Atlantic salmon populations show low levels of genetic differentiation relative to other salmonid species, when surveyed by allozymes, and with mitochondrial DNA and nuclear ribosomal DNA markers. Here we report the application of three novel microsatellite VNTR loci to population differentiation in...
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1995
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Online Access: | http://dx.doi.org/10.1139/f95-779 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-779 |
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crcansciencepubl:10.1139/f95-779 2023-12-17T10:27:08+01:00 Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations McConnell, Stewart K. O'Reilly, Patrick Hamilton, Lorraine Wright, Jonathan M. Bentzen, Paul 1995 http://dx.doi.org/10.1139/f95-779 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-779 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 52, issue 9, page 1863-1872 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1995 crcansciencepubl https://doi.org/10.1139/f95-779 2023-11-19T13:38:45Z Atlantic salmon populations show low levels of genetic differentiation relative to other salmonid species, when surveyed by allozymes, and with mitochondrial DNA and nuclear ribosomal DNA markers. Here we report the application of three novel microsatellite VNTR loci to population differentiation in Atlantic salmon. A total of 232 microsatellites, cloned from Atlantic salmon, were classified as perfect, imperfect, and compound repeats. Microsatellite length, as in other teleosts, was significantly larger than published mammalian microsatellites. Primers for PCR amplification of three salmon microsatellites were designed. Allele frequencies, degree of polymorphism, and heterozygosity were estimated for five populations from Nova Scotia, Canada, and from Europe. Nei's genetic distances of 0.02–0.9 were observed among populations. There was a clear discrimination between Canadian and European fish based on unique alleles present at two loci. These Atlantic salmon primers also amplify presumably homologous loci in nine other salmonid species. The polymorphic microsatellites loci reported here demonstrate great potential as genetic markers in population, breeding, and evolutionary studies. Article in Journal/Newspaper Atlantic salmon Salmo salar Canadian Science Publishing (via Crossref) Canada Canadian Journal of Fisheries and Aquatic Sciences 52 9 1863 1872 |
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Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics McConnell, Stewart K. O'Reilly, Patrick Hamilton, Lorraine Wright, Jonathan M. Bentzen, Paul Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Atlantic salmon populations show low levels of genetic differentiation relative to other salmonid species, when surveyed by allozymes, and with mitochondrial DNA and nuclear ribosomal DNA markers. Here we report the application of three novel microsatellite VNTR loci to population differentiation in Atlantic salmon. A total of 232 microsatellites, cloned from Atlantic salmon, were classified as perfect, imperfect, and compound repeats. Microsatellite length, as in other teleosts, was significantly larger than published mammalian microsatellites. Primers for PCR amplification of three salmon microsatellites were designed. Allele frequencies, degree of polymorphism, and heterozygosity were estimated for five populations from Nova Scotia, Canada, and from Europe. Nei's genetic distances of 0.02–0.9 were observed among populations. There was a clear discrimination between Canadian and European fish based on unique alleles present at two loci. These Atlantic salmon primers also amplify presumably homologous loci in nine other salmonid species. The polymorphic microsatellites loci reported here demonstrate great potential as genetic markers in population, breeding, and evolutionary studies. |
format |
Article in Journal/Newspaper |
author |
McConnell, Stewart K. O'Reilly, Patrick Hamilton, Lorraine Wright, Jonathan M. Bentzen, Paul |
author_facet |
McConnell, Stewart K. O'Reilly, Patrick Hamilton, Lorraine Wright, Jonathan M. Bentzen, Paul |
author_sort |
McConnell, Stewart K. |
title |
Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
title_short |
Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
title_full |
Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
title_fullStr |
Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
title_full_unstemmed |
Polymorphic microsatellite loci from Atlantic salmon ( Salmo salar ): genetic differentiation of North American and European populations |
title_sort |
polymorphic microsatellite loci from atlantic salmon ( salmo salar ): genetic differentiation of north american and european populations |
publisher |
Canadian Science Publishing |
publishDate |
1995 |
url |
http://dx.doi.org/10.1139/f95-779 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-779 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 52, issue 9, page 1863-1872 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f95-779 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
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52 |
container_issue |
9 |
container_start_page |
1863 |
op_container_end_page |
1872 |
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1785578909045096448 |